Document Detail

Inhibition of luteinizing hormone-human chorionic gonadotropin binding by retinoids in a Leydig cell line.
MedLine Citation:
PMID:  1820968     Owner:  NLM     Status:  MEDLINE    
Treatment of K9 mouse Leydig cells with 3 x 10(-6) M retinol (R) and retinoic acid (RA) resulted in 75% and 65% reduction of 125I-labeled hCG binding respectively, when assayed at 35 degrees C. This effect was dose-dependent and was first detected 12 h after initiation of treatment: it was maximal at 48 h for RA. R and RA had no significant effect on the rate of internalization and degradation of 125I-hCG as measured by disappearance of acid-releasable (i.e. surface-bound) radioactivity from the cells and by the appearance of trichloracetic acid-soluble label in the medium. When exposed to increasing concentrations of hCG for 24 h, both retinoid-treated and control cells 'down-regulated' their gonadotropin receptors with the same dose-dependent pattern. The kinetics of reappearance of the receptors was similar for retinoid-treated and control cells, but for treated cells the maximal number of receptors reinitiated at 24 h never exceeded 40% of the values observed with control cells. Scatchard plot analysis confirmed a decrease in hCG receptor number from approximately 26,000 to approximately 6400 and approximately 3500 sites per cell after R and RA treatment. Kd values for 125I-hCG binding were 2 x 10(-10) M, 7.3 x 10(-11) M and 6.9 x 10(-11) M for control, R- and RA-treated cells respectively. On the basis of our data it is likely that retinoid-induced reduction in 125I-hCG binding to K9 Leydig cells is due to decreased receptor synthesis.
A Lefèvre; C Astraudo; C Finaz
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular and cellular endocrinology     Volume:  76     ISSN:  0303-7207     ISO Abbreviation:  Mol. Cell. Endocrinol.     Publication Date:  1991 Apr 
Date Detail:
Created Date:  1992-08-11     Completed Date:  1992-08-11     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7500844     Medline TA:  Mol Cell Endocrinol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  115-23     Citation Subset:  IM    
Institut National de la Santé et de la Recherche Médicale, Unité 293, Montrouge, France.
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MeSH Terms
Cell Division
Cells, Cultured
Chorionic Gonadotropin / antagonists & inhibitors*
Leydig Cells / cytology,  metabolism*
Luteinizing Hormone / antagonists & inhibitors*
Protein Binding
Receptors, LH / antagonists & inhibitors
Tretinoin / pharmacology*
Vitamin A / pharmacology*
Reg. No./Substance:
0/Chorionic Gonadotropin; 0/Receptors, LH; 11103-57-4/Vitamin A; 302-79-4/Tretinoin; 9002-67-9/Luteinizing Hormone

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