Document Detail


Inhibition of ionophore-stimulated leukotriene B4 production in human leucocytes by monohydroxy fatty acids.
MedLine Citation:
PMID:  2994785     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Leukotriene B4 (LTB4) release by calcium ionophore-stimulated human leucocytes was measured by use of selective solvent partition of reaction mixtures and an agarose microdroplet chemokinesis assay, and the inhibitory effects of four monohydroxy fatty acids were determined. 15-Hydroxy-eicosatetraenoic acid (15-HETE) was the most effective inhibitor of LTB4 production with an approximate IC50 value of 6 microM and 99% inhibition at 50 microM, whereas 13-hydroxy-octadecadienoic acid (13-HODD) and 12-HETE were weaker inhibitors with approximate IC50 values of 32 microM and 23 microM, and 59% and 68% inhibition at 50 microM, respectively. We suggest that 13-HODD and 12-HETE, which are present in large amounts in the lesions of the skin disease psoriasis, may act as endogenous modulators of 5-lipoxygenase activity in skin.
Authors:
R D Camp; N J Fincham
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  British journal of pharmacology     Volume:  85     ISSN:  0007-1188     ISO Abbreviation:  Br. J. Pharmacol.     Publication Date:  1985 Aug 
Date Detail:
Created Date:  1985-11-08     Completed Date:  1985-11-08     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7502536     Medline TA:  Br J Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  837-41     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
Arachidonate Lipoxygenases
Calcimycin / pharmacology
Chromatography, High Pressure Liquid
Humans
Hydroxy Acids / pharmacology*
Hydroxyeicosatetraenoic Acids / pharmacology
Leukocytes / drug effects,  metabolism*
Leukotriene B4 / biosynthesis*
Linoleic Acids / pharmacology
Linoleic Acids, Conjugated*
Lipoxygenase / metabolism
Skin / enzymology
Time Factors
Chemical
Reg. No./Substance:
0/Hydroxy Acids; 0/Hydroxyeicosatetraenoic Acids; 0/Linoleic Acids; 0/Linoleic Acids, Conjugated; 15514-85-9/9-hydroxy-10,12-octadecadienoic acid; 5204-88-6/13-hydroxy-9,11-octadecadienoic acid; 52665-69-7/Calcimycin; 59985-28-3/12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; 71160-24-2/Leukotriene B4; 73945-47-8/15-hydroxy-5,8,11,13-eicosatetraenoic acid; EC 1.13.11.-/Arachidonate Lipoxygenases; EC 1.13.11.12/Lipoxygenase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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