Document Detail


Inhibition of intimal hyperplasia by photodynamic therapy using photofrin.
MedLine Citation:
PMID:  1494287     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Photodynamic therapy using photofrin and light energy inhibits human myofibroblast proliferation in cell culture. The purpose of this study is to evaluate its influence on intimal hyperplasia in vivo. Twenty New Zealand White rabbits underwent a standardized intimal injury to both common carotid arteries with a 2 Fr balloon catheter. One week later, half of the animals received photofrin (5 mg/kg) intravenously. The remaining 10 rabbits received no photofrin. Two days later, all neck incisions were reopened and a 1-cm segment of each of the 40 carotid arteries was exposed for 5 min to 80 mW of 630 nm light energy from a continuous wave tunable dye laser (fluence = 7.6 J/cm2). All vessels were harvested 5 weeks post-laser treatment following in vivo fixation with formalin. From each artery, separate cross-sections taken from both the lasered and non-lasered regions of each vessel were mounted and stained for histologic evaluation. Analyzed segments were then divided into four different treatment groups: group I segments consisted of arterial cross-sections which were taken from vessel regions that were injured but received neither photofrin nor laser treatment (group I, n = 20); group II segments also did not receive photofrin but were exposed to light energy (group II, n = 20); group III segments received photofrin but no light energy (group III, n = 20); and cross-sections in group IV were taken from those segments which received both photofrin and laser treatment. Using planimetry, the ratio of the area of intimal hyperplasia (IH) to the area enclosed by the internal elastic lamina (IEL) was measured for each specimen (IH/IEL).(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
D Eton; M D Colburn; V Shim; W Panek; D Lee; W S Moore; S S Ahn
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of surgical research     Volume:  53     ISSN:  0022-4804     ISO Abbreviation:  J. Surg. Res.     Publication Date:  1992 Dec 
Date Detail:
Created Date:  1993-03-10     Completed Date:  1993-03-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  558-62     Citation Subset:  IM    
Affiliation:
Department of Surgery, UCLA Center for the Health Sciences 90024-6904.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carotid Arteries / drug effects*,  pathology
Dihematoporphyrin Ether / pharmacology*
Endothelium, Vascular / drug effects*,  pathology
Hyperplasia
Necrosis
Photochemotherapy*
Rabbits
Chemical
Reg. No./Substance:
97067-70-4/Dihematoporphyrin Ether

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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