Document Detail

Inhibition of inducible nitric oxide synthase in vitro and in vivo by a water-soluble extract of Wendita calysina leaves.
MedLine Citation:
PMID:  17619177     Owner:  NLM     Status:  MEDLINE    
Wendita calysina is a Paraguayan herbaceous plant commonly known as burrito. Our previous study indicated that burrito leaves are a very good source of phenylpropanoid glycosides, principally verbascoside. From W. calysina leaves, a standardized, water-soluble extract (WSE) rich in phenylpropanoid glycosides has been developed on an industrial scale to be used as a food supplement, cosmetic, phytomedicine, and ingredient of different formulations. In this study, we investigated the effect of the W. calysina WSE both in vitro in murine macrophage cell line J774.A1 stimulated with lipopolysaccharide (LPS) and, in vivo in an animal model of acute inflammation, carrageenan-induced pleurisy. Here we report that W. calysina WSE (0.05, 0.1, and 0.5 mg/ml) inhibited inducible nitric oxide synthase (iNOS) expression and activity in LPS-stimulated J774.A1. In vivo experiments showed that injection of carrageenan (2%) into the pleural cavity of rats elicited an acute inflammatory response characterized by iNOS expression, intercellular adhesion molecule-1 (ICAM-1) up-regulation, nitrotyrosine and poly (ADP-ribose) synthase (PARS) formation, and lung tissue damage-all parameters significantly reduced by W. calysina WSE (500 mg/kg per os). These results report, for the first time, that a treatment with W. calysina WSE exerts anti-inflammatory effects both in vitro and in vivo.
Stefania Marzocco; Anna Lisa Piccinelli; Luca Rastrelli; Emanuela Mazzon; Salvatore Cuzzocrea; Giuseppina Autore
Related Documents :
17078937 - Platelet nitric oxide synthesis in uremia and malnutrition: a role for l-arginine suppl...
7509287 - Effect of ng-nitro-l-arginine on shock induced by endotoxin and by platelet activating ...
10837347 - Bioactive products of arginine in sepsis: tissue and plasma composition after lps and i...
11035207 - Opposing interplay between neuropeptide ff and nitric oxide in antinociception and hypo...
15765777 - The role of autoreactive t-cells in the pathogenesis of idiopathic thrombocytopenic pur...
9688597 - Topological analysis of nhe1, the ubiquitous na+/h+ exchanger using chymotryptic cleavage.
Publication Detail:
Type:  In Vitro; Journal Article     Date:  2007-07-07
Journal Detail:
Title:  Naunyn-Schmiedeberg's archives of pharmacology     Volume:  375     ISSN:  0028-1298     ISO Abbreviation:  Naunyn Schmiedebergs Arch. Pharmacol.     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-07-19     Completed Date:  2007-12-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0326264     Medline TA:  Naunyn Schmiedebergs Arch Pharmacol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  349-58     Citation Subset:  IM    
Department of Pharmaceutical Sciences, School of Pharmacy, University of Salerno, Via Ponte don Melillo, 84084 Fisciano, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Anti-Inflammatory Agents / isolation & purification,  pharmacology*
Cell Line
Cell Survival / drug effects
Dose-Response Relationship, Drug
Exudates and Transudates / cytology,  metabolism
Geraniaceae / chemistry*
Intercellular Adhesion Molecule-1 / biosynthesis
Lipopolysaccharides / pharmacology
Neutrophils / pathology
Nitric Oxide / biosynthesis
Nitric Oxide Synthase Type II / antagonists & inhibitors*,  biosynthesis
Plant Extracts / isolation & purification,  pharmacology
Plant Leaves / chemistry*
Pleurisy / chemically induced,  metabolism,  pathology
Poly Adenosine Diphosphate Ribose / metabolism
Rats, Sprague-Dawley
Tyrosine / analogs & derivatives,  biosynthesis
Reg. No./Substance:
0/Anti-Inflammatory Agents; 0/Lipopolysaccharides; 0/Plant Extracts; 10102-43-9/Nitric Oxide; 126547-89-5/Intercellular Adhesion Molecule-1; 26656-46-2/Poly Adenosine Diphosphate Ribose; 3604-79-3/3-nitrotyrosine; 55520-40-6/Tyrosine; 9000-07-1/Carrageenan; EC Oxide Synthase Type II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  School marks for Swedish children whose mothers had diabetes during pregnancy: a population-based st...
Next Document:  Effects of pregnancy on nicotine self-administration and nicotine pharmacokinetics in rats.