|Inhibition of inducible nitric oxide synthase prevents hepatic, but not pulmonary, injury following ischemia-reperfusion of rat liver.|
|PMID: 16614969 Owner: NLM Status: MEDLINE|
|The aim of this study was to investigate the contribution of inducible nitric oxide synthase (iNOS)-derived nitric oxide on the liver and lung injury following hepatic ischemia-reperfusion (I/R) using a novel and potent iNOS inhibitor, ONO-1714. Rats were subjected to 90 min of partial hepatic ischemia followed by 3, 6, 12, and 24 hr of reperfusion. Expression of iNOS mRNA peaked at 3 hr of reperfusion in the liver and lung. Plasma nitric oxide levels were increased fourfold at 24 hr of reperfusion and plasma ALT was increased, reaching a peak at 12 hr of reperfusion; both were significantly inhibited by ONO-1714. Histological examination revealed extensive liver damage, whereas this was not seen in the ONO-1714 group. Lung injury was not significantly changed in groups with versus without ONO-1714. Nitrotyrosine expression was seen in regions similar to those of the histological injuries of the liver, while this staining was absent in the ONO-1714 group. These data show that generation of peroxynitrite could be involved in the pathogenesis of liver injury but not lung injury after hepatic I/R. Inhibition of iNOS could be applied for attenuation of liver injury following hepatic I/R.|
|Yuji Takamatsu; Kazuo Shimada; Koji Yamaguchi; Syoji Kuroki; Kazuo Chijiiwa; Masao Tanaka|
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|Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't|
|Title: Digestive diseases and sciences Volume: 51 ISSN: 0163-2116 ISO Abbreviation: Dig. Dis. Sci. Publication Date: 2006 Mar|
|Created Date: 2006-04-14 Completed Date: 2006-05-17 Revised Date: 2006-11-15|
Medline Journal Info:
|Nlm Unique ID: 7902782 Medline TA: Dig Dis Sci Country: United States|
|Languages: eng Pagination: 571-9 Citation Subset: AIM; IM|
|Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.|
|APA/MLA Format Download EndNote Download BibTex|
Analysis of Variance
Disease Models, Animal
Heterocyclic Compounds, 2-Ring / pharmacology
Ischemia / complications, therapy
Liver / blood supply*
Liver Diseases / prevention & control*
Lung Diseases / prevention & control*
Molecular Sequence Data
Nitric Oxide Synthase Type II / drug effects*, metabolism
RNA, Messenger / analysis
Reperfusion / methods
Reperfusion Injury / prevention & control*
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity
|0/7-chloro-3-imino-5-methyl-2-azabicyclo(4.1.0)heptane; 0/Amidines; 0/Heterocyclic Compounds, 2-Ring; 0/RNA, Messenger; EC 188.8.131.52/Nitric Oxide Synthase Type II|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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