Document Detail

Inhibition of in-vitro fertilization of intact and denuded hamster eggs by univalent anti-sperm antibodies.
MedLine Citation:
PMID:  439079     Owner:  NLM     Status:  MEDLINE    
Univalent (Fab) rabbit anti-hamster sperm antibodies added to an in-vitro fertilization system did not interfere with the sperm acrosome reaction or motility, but inhibited cumulus dispersion by the spermatozoa, sperm binding to and passage through the zona pellucida as well as sperm-egg fusion. Addition of the Fab preparations to the capacitated spermatozoa at various times before or up to 40-45 min after the sperm-egg mixing prevented penetration of spermatozoa through the zona pellucida. Detachment of the spermatozoa already bound as well as those partly inside the zona pellucida was achieved by a late addition of antibodies. In experiments with zona-free hamster eggs, addition of the Fab antibodies to the spermatozoa 10 min to 5 h before the introduction of unfertilized eggs reduced the rate of adhesion and fertilization to very low levels. These antibodies were not absorbed on hamster ovary, liver or kidney and had no direct effect on the fertilizability of zona-intact or zona-free eggs.
S J Tzartos
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of reproduction and fertility     Volume:  55     ISSN:  0022-4251     ISO Abbreviation:  J. Reprod. Fertil.     Publication Date:  1979 Mar 
Date Detail:
Created Date:  1979-07-28     Completed Date:  1979-07-28     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0376367     Medline TA:  J Reprod Fertil     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  447-55     Citation Subset:  IM    
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MeSH Terms
Fertilization in Vitro* / methods
Immunoglobulin Fab Fragments / immunology
Ovum / immunology*
Sperm Agglutination
Sperm Motility / drug effects
Spermatozoa / immunology*
Zona Pellucida / immunology*
Reg. No./Substance:
0/Antibodies; 0/Immunoglobulin Fab Fragments

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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