Document Detail

Inhibition of immune cell proliferation with haloperidol and relationship of tyrosine hydroxylase expression to immune cell growth.
MedLine Citation:
PMID:  9619850     Owner:  NLM     Status:  MEDLINE    
Previous studies indicated that exogenous dopamine and its agonists directly regulated mitogen-induced immune cell proliferation. In this study, we further investigated role of endogenous dopamine in immune cell growth. Haloperidol, a general antagonist for dopamine receptors, could reduce the cell growth rate of T cell hybridoma (10I) and rat nervous pheochromocytoma cells (PC12). Tyrosine hydroxylase (TH) catalyzes the initial rate-limiting step of catecholamine biosynthesis in the nervous system. Flow cytometric analysis indicated the expression of TH in various immune cells. The presence of TH in PC12 cells was used as a control. Temporal studies indicated that the expression of TH increased during 10I cell growth. Both alpha-methyl-p-tyrosine and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine reduced TH expression and cell growth in a dose-dependent manner. These results suggest that immune T cells express TH which is correlated to cell growth, and that dopamine released from these cells may bind to the receptors to act in an autocrine or paracrine way.
C W Tsao; Y S Lin; J T Cheng
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Life sciences     Volume:  62     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  1998  
Date Detail:
Created Date:  1998-06-18     Completed Date:  1998-06-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  PL 335-44     Citation Subset:  IM    
Department of Pharmacology, National Cheng Kung University Medical College, Tainan, Taiwan.
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MeSH Terms
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine / pharmacology
Cell Division / drug effects*
Dopamine Agents / pharmacology
Dopamine Antagonists / pharmacology*
Enzyme Inhibitors / pharmacology
Flow Cytometry
Gene Expression* / drug effects
Haloperidol / pharmacology*
PC12 Cells / cytology
T-Lymphocytes / cytology*
Tumor Cells, Cultured
Tyrosine 3-Monooxygenase / antagonists & inhibitors,  genetics*
alpha-Methyltyrosine / pharmacology
Reg. No./Substance:
0/Dopamine Agents; 0/Dopamine Antagonists; 0/Enzyme Inhibitors; 28289-54-5/1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; 52-86-8/Haloperidol; 658-48-0/alpha-Methyltyrosine; EC 3-Monooxygenase

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