| Inhibition of human lung adenocarcinoma growth using survivint34a by low-dose systematic administration. | |
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MedLine Citation:
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PMID: 20689177 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Anti-apoptosis plays an important role in tumour formation and development. Survivin is a member of the inhibitor of apoptosis (IAP) family, which is a target for anti-cancer drug exploitation was replaced as development. We investigated the role of the homo dominant-negative mutant Survivin-T34A in suppressing human lung adenocarcinomas (A549). The anti-tumour activity of HSurvivinT34A plasmid was evaluated in the A549 cell line and nude mice bearing A549 subcutaneous tumours. Low-dose systemic administration was continuously used. The HSurvivinT34A plasmid (5 meu g/one) complexed with a cationic liposome (DOTAP/Chol) significantly inhibited tumour growth in our model. We observed microvessel density degradation by CD31 immunohistochemistry and apoptotic cell increase by TUNEL assay, PI staining and flow cytometric analysis in the treated group. The present findings suggest that the HSurvivinT34A plasmid complexed with a cationic liposome may provide an effective approach to inhibit the growth of human lung adenocarcinomas in vitro and in vivo. |
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Authors:
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Yan Shan; Chunting Wang; Li Yang; Li Juan Chen; Hong Xin Deng; Han Shuo Yang; Zhimian Li; Zhiyong Li; Li Pan; Fei Leng; Yuquan Wei |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biosciences Volume: 35 ISSN: 0973-7138 ISO Abbreviation: J. Biosci. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-08-06 Completed Date: 2011-01-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8100809 Medline TA: J Biosci Country: India |
Other Details:
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Languages: eng Pagination: 209-16 Citation Subset: IM |
Affiliation:
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State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Keyuan Road 4, Chengdu, Sichuan, 610041, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
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pathology,
therapy* Animals Cell Line, Tumor Female Gene Therapy Gene Transfer Techniques Humans Lung Neoplasms / pathology, therapy* Mice Mice, Inbred BALB C Mice, Nude Microtubule-Associated Proteins / genetics* Neoplasm Transplantation Plasmids Xenograft Model Antitumor Assays |
| Chemical | |
Reg. No./Substance:
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0/BIRC5 protein, human; 0/Microtubule-Associated Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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