Document Detail

Inhibition of growth and increase of alkaline phosphatase activity in cultured human oral cancer cells by all-trans retinoic acid.
MedLine Citation:
PMID:  16497480     Owner:  NLM     Status:  MEDLINE    
In this study, the effects of all-trans retinoic acid (ATRA) on human oral cancer cells with regard to cell growth, the cell cycle, and alkaline phosphatase (ALP) activity were evaluated. Human oral cancer KB cells were treated with various concentrations of ATRA, and cell growth was then determined using the MTT viability assay. The cell-cycle distribution and ALP activity were analysed using a flow cytometer and chemical analyser, respectively. The KB cells were inhibited by ATRA at concentrations of 1-16 microM (1 microM, P<0.05; 2 microM, P<0.01; 4, 8 and 16 microM, P<0.001) in a dose-dependent manner. ATRA arrested KB cells in the G0/G1 phase. The ALP activity in KB cells was increased by ATRA. This is one of the first studies to focus on the expression of ALP in human head-and-neck carcinoma cells treated with retinoids. These findings suggest that the anti-tumour effects of ATRA on human oral cancer are associated with G0/G1 phase arrest and an increase in ALP activity.
B Xiao; J Guo; Y Lou; D Meng; W Zhao; L Zhang; C Yan; D Wang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-02-23
Journal Detail:
Title:  International journal of oral and maxillofacial surgery     Volume:  35     ISSN:  0901-5027     ISO Abbreviation:  Int J Oral Maxillofac Surg     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-06-19     Completed Date:  2006-08-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8605826     Medline TA:  Int J Oral Maxillofac Surg     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  643-8     Citation Subset:  D; IM    
School of Medicine, Ningbo University, Ningbo 315211, China.
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MeSH Terms
Alkaline Phosphatase / biosynthesis
Antineoplastic Agents / pharmacology*,  therapeutic use
Apoptosis / drug effects
Carcinoma, Squamous Cell / drug therapy*,  enzymology
Dose-Response Relationship, Drug
Flow Cytometry
G0 Phase / drug effects*
G1 Phase / drug effects*
KB Cells / drug effects
Mouth Neoplasms / drug therapy*,  enzymology
Tretinoin / pharmacology*,  therapeutic use
Reg. No./Substance:
0/Antineoplastic Agents; 302-79-4/Tretinoin; EC Phosphatase

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