Document Detail


Inhibition of gonadotropin-responsive adenylate cyclase in MA-10 Leydig tumor cells by epidermal growth factor.
MedLine Citation:
PMID:  2454919     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have previously shown that mouse epidermal growth factor (mEGF) attenuates the increase in intracellular cAMP provoked by human choriogonadotropin (hCG) in MA-10 Leydig tumor cells (Ascoli, M., Euffa, J., and Segaloff, D. L. (1987) J. Biol. Chem. 262, 9196-9203). The studies presented herein were designed to investigate the mechanism(s) responsible for this phenomenon. We show that mEGF attenuates the increase in cAMP accumulation provoked by hCG primarily, if not entirely, by inhibiting adenylate cyclase activity. This phenomenon has some specificity for the agonist used, but it is not cell-specific. Thus, mEGF inhibited hCG-activated adenylate cyclase in MA-10 cells and in rat luteal cells but had no effect on the forskolin-activated enzyme in MA-10 cells or the isoproterenol-activated enzyme in rat luteal cells.
Authors:
M E Pereira; D L Segaloff; M Ascoli
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  263     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1988 Jul 
Date Detail:
Created Date:  1988-08-08     Completed Date:  1988-08-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  9761-6     Citation Subset:  IM    
Affiliation:
Population Council, New York, New York 10021.
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MeSH Terms
Descriptor/Qualifier:
1-Methyl-3-isobutylxanthine / pharmacology
Adenylate Cyclase / antagonists & inhibitors*,  metabolism
Animals
Chorionic Gonadotropin / pharmacology*
Cyclic AMP / biosynthesis
Enzyme Activation / drug effects
Epidermal Growth Factor / pharmacology*
Female
Forskolin / pharmacology
Humans
Isoproterenol / pharmacology
Leydig Cell Tumor / enzymology*
Luteal Cells / enzymology
Mice
Rats
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
CA-40629/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Chorionic Gonadotropin; 28822-58-4/1-Methyl-3-isobutylxanthine; 60-92-4/Cyclic AMP; 62229-50-9/Epidermal Growth Factor; 66428-89-5/Forskolin; 7683-59-2/Isoproterenol; EC 4.6.1.1/Adenylate Cyclase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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