| Inhibition of gonadotropin-responsive adenylate cyclase in MA-10 Leydig tumor cells by epidermal growth factor. | |
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MedLine Citation:
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PMID: 2454919 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have previously shown that mouse epidermal growth factor (mEGF) attenuates the increase in intracellular cAMP provoked by human choriogonadotropin (hCG) in MA-10 Leydig tumor cells (Ascoli, M., Euffa, J., and Segaloff, D. L. (1987) J. Biol. Chem. 262, 9196-9203). The studies presented herein were designed to investigate the mechanism(s) responsible for this phenomenon. We show that mEGF attenuates the increase in cAMP accumulation provoked by hCG primarily, if not entirely, by inhibiting adenylate cyclase activity. This phenomenon has some specificity for the agonist used, but it is not cell-specific. Thus, mEGF inhibited hCG-activated adenylate cyclase in MA-10 cells and in rat luteal cells but had no effect on the forskolin-activated enzyme in MA-10 cells or the isoproterenol-activated enzyme in rat luteal cells. |
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Authors:
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M E Pereira; D L Segaloff; M Ascoli |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 263 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1988 Jul |
Date Detail:
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Created Date: 1988-08-08 Completed Date: 1988-08-08 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 9761-6 Citation Subset: IM |
Affiliation:
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Population Council, New York, New York 10021. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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1-Methyl-3-isobutylxanthine
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pharmacology Adenylate Cyclase / antagonists & inhibitors*, metabolism Animals Chorionic Gonadotropin / pharmacology* Cyclic AMP / biosynthesis Enzyme Activation / drug effects Epidermal Growth Factor / pharmacology* Female Forskolin / pharmacology Humans Isoproterenol / pharmacology Leydig Cell Tumor / enzymology* Luteal Cells / enzymology Mice Rats Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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CA-40629/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chorionic Gonadotropin; 28822-58-4/1-Methyl-3-isobutylxanthine; 60-92-4/Cyclic AMP; 62229-50-9/Epidermal Growth Factor; 66428-89-5/Forskolin; 7683-59-2/Isoproterenol; EC 4.6.1.1/Adenylate Cyclase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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