| Inhibition of genotoxic stress induced apoptosis by novel TAT-fused peptides targeting PIDDosome. | |
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MedLine Citation:
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PMID: 22056621 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Genotoxic stress induced apoptosis is mediated by the formation of PIDDosome, which is a caspase-2 activating complex composed of three protein components, PIDD, RAIDD, and caspase-2. Here, synthetic TAT-fused peptides designed by the structure of PIDD and RAIDD, TAT-Y814A and TAT-R147E, respectively, were produced and tested for their ability to inhibit PIDDosome formation in vitro as well as to attenuate genotoxic stress-induced apoptosis in human renal cancer cells. The results show that TAT-Y814A and TAT-R147E have the potential to inhibit formation of the PIDDosome in a dose-dependent manner. Furthermore, both peptides partially inhibit genotoxic stress mediated apoptosis and activation of caspase2 and caspase3 in Caki cells. These results suggest that TAT-Y814A (also TAT-R147E) is a novel inhibitor of genotoxic stress-induced apoptosis that may serve as a prototype for anti-apoptotic drug development. |
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Authors:
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Tae-Ho Jang; Sung-Jun Lee; Chang-Hoon Woo; Kyung Jin Lee; Ju-Hong Jeon; Dong-Sup Lee; Kihang Choi; In-Gyu Kim; Young Whan Kim; Tae-Jin Lee; Hyun Ho Park |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-10-25 |
Journal Detail:
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Title: Biochemical pharmacology Volume: - ISSN: 1873-2968 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-11-7 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0101032 Medline TA: Biochem Pharmacol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011. Published by Elsevier Inc. |
Affiliation:
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Graduate School of Biochemistry at Yeungnam University, Gyeongsan, South Korea. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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