Document Detail

Inhibition of gap junction-mediated intercellular communication by alpha-linolenate.
MedLine Citation:
PMID:  1677533     Owner:  NLM     Status:  MEDLINE    
The purpose of this investigation was to assess whether alterations in the fatty acid composition of rat liver epithelial (WB-F344) cell phospholipids would modulate gap junction-mediated intercellular communication (GJIC). WB-F344 cells were grown to confluency in culture medium supplemented with one of seven different fatty acids at a concentration of 50 microM for 48 h. Only alpha-linoleate (18:3 n-3) significantly inhibited GJIC. Saturated fatty acids (12:0, 16:0, and 18:0), a monounsaturated fatty acid (18:1 n-9), and n-6 polyunsaturated fatty acids (18:2 and 20:4) did not affect GJIC. The alpha-linolenate-induced inhibition of GJIC was not due to the activation of protein kinase C or intracellular hydroperoxide production, two lipid-dependent parameters previously shown to inhibit GJIC. In addition, alpha-linolenate did not alter membrane fluidity. Although the mechanism by which alpha-linolenate inhibits GJIC is unclear, changes in the fatty acid composition of cell phospholipids may be of critical importance. Subsequent to supplementation with alpha-linolenate, WB-F344 cell phospholipids had reduced 20:4 n-6 and elevated n-3 fatty acids. The results of this investigation emphasize the importance of current research into the influence of lipids on cell function and identify a new mechanism by which gap junctions can be modulated.
C M Hasler; M R Bennink; J E Trosko
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  261     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1991 Jul 
Date Detail:
Created Date:  1991-08-27     Completed Date:  1991-08-27     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  C161-8     Citation Subset:  IM    
Department of Food Science and Human Nutrition, Michigan State University, East Lansing 48824.
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MeSH Terms
Cell Communication*
Cell Line
Fatty Acids / metabolism
Intercellular Junctions / metabolism*
Linolenic Acids / metabolism*
Peroxides / metabolism
Phospholipids / metabolism
Protein Kinase C / metabolism
alpha-Linolenic Acid
Grant Support
1P42-ES-04911-01/ES/NIEHS NIH HHS
Reg. No./Substance:
0/Fatty Acids; 0/Linolenic Acids; 0/Peroxides; 0/Phospholipids; 463-40-1/alpha-Linolenic Acid; EC Kinase C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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