Document Detail

Inhibition of erythrocyte sickling by cystamine, a thiol reagent.
MedLine Citation:
PMID:  135260     Owner:  NLM     Status:  MEDLINE    
Incubation of sickle cells with cystamine, a thiol reagnet, resulted in the formation of an intracellular S-ethylamine derivative. The rate of the reaction was dependent upon the cystamine concentration, the temperature, and the duration of the incubation. The cystamine-treated cells demonstrated a marked inhibition of sickling under hypoxic conditions, a decrease in their mean corpuscular hemoglobin concentration, and a significant increase in their oxygen affinity. The oxygen affinity of these cells was less dependent on their mean corpuscular hemoglobin concentration than that of untreated sickle cells. The minimum gelling concentration of S-ethylamine doxyhemoglobin S was slightly increased. Cystamine did not affect the intracellular pH nor the 2,3-diphosphoglycerate level. The exact contribution of the interrelated factors in cystamine inhibition of sickling (changes in oxygen affinity, mean corpuscular hemoglobin concentration, and minimum gelling concentration) has yet to be determined.
W Hassan; Y Beuzard; J Rosa
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  73     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1976 Sep 
Date Detail:
Created Date:  1976-12-01     Completed Date:  1976-12-01     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3288-92     Citation Subset:  IM    
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MeSH Terms
Anemia, Sickle Cell / blood
Cystamine / pharmacology*
Erythrocytes / enzymology
Erythrocytes, Abnormal / drug effects*
Oxygen Consumption
Phosphofructokinase-1 / antagonists & inhibitors
Phytic Acid / pharmacology
Protein Conformation / drug effects
Pyruvate Kinase / antagonists & inhibitors
Sickle Cell Trait / blood
Reg. No./Substance:
0/Gels; 51-85-4/Cystamine; 83-86-3/Phytic Acid; EC; EC Kinase

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