| Inhibition of epithelial to mesenchymal transition in metastatic breast carcinoma cells by c-Src suppression. | |
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MedLine Citation:
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PMID: 20705589 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aberrant activation of c-Src regulates multiple functions during tumor progression. This study was conducted to investigate the role of c-Src suppression in epithelial to mesenchymal transition (EMT) process in human breast carcinoma cells. c-Src suppression by PP2 (a Src-family kinase inhibitor) or small interfering RNA (siRNA) was carried out in MCF-7 and MDA-MB-231 cells. Cell migration was analyzed by wound-healing assay. The transcription and protein levels of EMT markers and transcription factors were evaluated by reverse transcription-PCR and Western blot analysis, respectively. The changed cell morphology was photographed by light microscope. c-Src suppression by PP2 or siRNA reversed the mesenchymal-like phenotype in MDA-MB-231 cells. E-cadherin was upregulated in MCF-7 and MDA-MB-231 cells after c-Src suppression, whereas vimentin was downregulated in MDA-MB-231 cells. Slug and SIP1 were downregulated after c-Src suppression in MCF-7 and MDA-MB-231 cells, whereas Twist was unchanged. These results suggest that c-Src suppression by PP2 or siRNA may inhibit EMT through regulation of different transcription factors in breast carcinoma cells that have different metastatic potential. |
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Authors:
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Xiang Liu; Renqing Feng |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-10 |
Journal Detail:
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Title: Acta biochimica et biophysica Sinica Volume: 42 ISSN: 1745-7270 ISO Abbreviation: Acta Biochim. Biophys. Sin. (Shanghai) Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-08-13 Completed Date: 2010-11-29 Revised Date: 2011-09-28 |
Medline Journal Info:
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Nlm Unique ID: 101206716 Medline TA: Acta Biochim Biophys Sin (Shanghai) Country: China |
Other Details:
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Languages: eng Pagination: 496-501 Citation Subset: IM |
Affiliation:
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Peking University, Beijing, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Blotting, Western Breast Neoplasms / genetics, metabolism, pathology Cadherins / genetics, metabolism Cell Line Cell Line, Tumor Cell Movement / drug effects Epithelial Cells / metabolism*, pathology Humans Mesoderm / metabolism*, pathology Neoplasm Metastasis Nerve Tissue Proteins / genetics, metabolism Pyrimidines / pharmacology RNA Interference RNA-Binding Proteins / genetics, metabolism Reverse Transcriptase Polymerase Chain Reaction Transcription Factors / genetics, metabolism Tumor Markers, Biological / genetics, metabolism* Vimentin / genetics, metabolism src-Family Kinases / antagonists & inhibitors, genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/AG 1879; 0/Cadherins; 0/GEMIN2 protein, human; 0/Nerve Tissue Proteins; 0/Pyrimidines; 0/RNA-Binding Proteins; 0/Transcription Factors; 0/Tumor Markers, Biological; 0/Vimentin; 0/snail family transcription factors; EC 2.7.10.2/src-Family Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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