| Inhibition of endothelial nitric oxide generation by low-density lipoprotein is partially prevented by L-arginine and L-ascorbate. | |
| | |
MedLine Citation:
|
PMID: 15380458 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We evaluated, in endothelial cells, the relative effectiveness of L-arginine and L-ascorbate in preventing the decrease in nitric oxide (NO) production in response to native low-density lipoprotein (LDL) from healthy subjects (nLDL), oxidized LDL (oxLDL, formed by nLDL oxidation) or native LDL from type 2 diabetic patients (dLDL). Human umbilical vein endothelial cells (HUVEC) were exposed to nLDL, dLDL or oxLDL (100 mg protein/L), in the absence or presence of L-arginine 10(-4)mol/L and/or L-ascorbate 10(-4)mol/L; NO synthase (NOS) activity and cyclic guanosine-3',5'-monophosphate (cGMP) were measured by the conversion of L-[3H]-arginine to L-[3H]citrulline and by radioimmunoassay, respectively. Both L-arginine and L-ascorbate increased cGMP in HUVEC co-incubated with any LDL species, although to lower levels than found in the absence of LDL. L-ascorbate did not affect NOS activity, whereas L-arginine increased it, both in the absence and presence of all LDL species. The effects of combined L-arginine and L-ascorbate on NOS activity and cGMP were no greater than those of L-arginine alone. Our results suggest that L-arginine or L-ascorbate can ameliorate, but not normalize, NO production in this situation, and that combining L-arginine with L-ascorbate is unlikely to produce additional benefit as compared with L-arginine alone. |
| | |
Authors:
|
Yong Ji; Yi Han; Jianxin Diao; Yan Huang; Qi Chen; Albert Ferro |
Related Documents
:
|
8795448 - Effects of inhaled nitric oxide compared with aspirin on platelet function in vivo in h... 9351518 - Nitroglycerin-inhibited whole blood aggregation is partially mediated by calcitonin gen... 1963618 - Formation of free radicals and nitric oxide derivative of hemoglobin in rats during sho... 9445268 - Endogenous nitric oxide protects against thromboembolism in venules but not in arterioles. 6166738 - Beta-thromboglobulin levels in plasma of jaundiced neonates exposed to phototherapy. 4359538 - Metabolism of sulfoquinovosyl diglyceride in chlorella pyrenoidosa by sulfoquinovosyl m... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Atherosclerosis Volume: 176 ISSN: 0021-9150 ISO Abbreviation: Atherosclerosis Publication Date: 2004 Oct |
Date Detail:
|
Created Date: 2004-09-21 Completed Date: 2005-01-24 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0242543 Medline TA: Atherosclerosis Country: Ireland |
Other Details:
|
Languages: eng Pagination: 345-53 Citation Subset: IM |
Affiliation:
|
Atherosclerosis Research Centre, Nanjing Medical University, Nanjing 210029, PR China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Arginine / pharmacology* Ascorbic Acid / pharmacology* Cyclic GMP / metabolism Diabetes Mellitus, Type 2 / complications Endothelial Cells Female Free Radical Scavengers / pharmacology* Humans Lipoproteins, LDL / pharmacology* Male Middle Aged Nitric Oxide / analysis* |
| Chemical | |
Reg. No./Substance:
|
0/Free Radical Scavengers; 0/Lipoproteins, LDL; 0/oxidized low density lipoprotein; 10102-43-9/Nitric Oxide; 50-81-7/Ascorbic Acid; 74-79-3/Arginine; 7665-99-8/Cyclic GMP |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Acute hyperhomocysteinemia decreases NO bioavailability in healthy adults.
Next Document: Potential of free-form TFPI and PAI-1 to be useful markers of early atherosclerosis in a Japanese ge...