Document Detail


Inhibition of endoplasm reticulum stress by ghrelin protects against ischemia/reperfusion injury in rat heart.
MedLine Citation:
PMID:  19406177     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ghrelin is a multi-functional polypeptide with cardiovascular protective effects. We aimed to explore whether the cardioprotective effect of ghrelin is mediated by inhibiting myocardial endoplasmic reticulum stress (ERS). A Langendorff model of isolated rat heart was used with ischemia/reperfusion (I/R; 40/120 min). Cardiac function was monitored, and histomorphologic features, degree of myocardial injury, level of ERS markers, and number of apoptotic cardiomyocytes were determined. Compared with control group, the I/R group showed significantly decreased cardiac function, seriously damaged myocardial tissue, increased number of apoptotic cells, and overexpression of mRNA and protein of ERS markers. However, preadministration of ghrelin in vivo (10(-8)mol/kg, intraperitoneal injection, every 12h, twice in all) greatly ameliorated the damaged heart function, attenuated myocardial injury and apoptosis, and decreased the expression of ERS markers: it decreased the mRNA and protein levels of glucose-regulated protein78 (GRP78) and C/EBP homologous protein (CHOP), with reduced caspase-12 protein expression. Furthermore, in vitro, ghrelin directly inhibited the myocardial ERS response induced by tunicamycin or dithiothreitol in rat cardiac tissue. Ghrelin could protect the heart against I/R injury, at least in part, through inhibiting myocardial ERS.
Authors:
Gai-Gai Zhang; Xu Teng; Yue Liu; Yan Cai; Ye-Bo Zhou; Xiao-Hui Duan; Jun-Qiu Song; Yi Shi; Chao-Shu Tang; Xin-Hua Yin; Yong-Fen Qi
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-05-03
Journal Detail:
Title:  Peptides     Volume:  30     ISSN:  1873-5169     ISO Abbreviation:  Peptides     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-05-25     Completed Date:  2009-09-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008690     Medline TA:  Peptides     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1109-16     Citation Subset:  IM    
Affiliation:
Department of Cardiology, the Second Affiliated Hospital of Harbin Medical University, Harbin, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects
Dithiothreitol / antagonists & inhibitors,  pharmacology
Endoplasmic Reticulum / drug effects*
Ghrelin / pharmacology*
Heart Rate / drug effects
Humans
Male
Myocardial Reperfusion Injury / metabolism,  pathology,  prevention & control*
Myocardium / metabolism*,  pathology
Rats
Rats, Sprague-Dawley
Tunicamycin / antagonists & inhibitors,  pharmacology
Ventricular Function, Left / drug effects
Chemical
Reg. No./Substance:
0/Ghrelin; 11089-65-9/Tunicamycin; 3483-12-3/Dithiothreitol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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