Document Detail

Inhibition of endogenous ganglioside synthesis does not block neurite formation by retinoic acid-treated neuroblastoma cells.
MedLine Citation:
PMID:  8995443     Owner:  NLM     Status:  MEDLINE    
Gangliosides are believed to play a critical role in cellular differentiation. To test this concept, we determined the effect of inhibition of endogenous ganglioside synthesis upon neurite formation induced by retinoic acid in LAN-5 human neuroblastoma cells. Ganglioside synthesis and content of LAN-5 cells exposed for 6 days to 10 microM D-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol (D-PDMP) (an inhibitor of glucosylceramide synthase) were reduced by >90%. However, these ganglioside-depleted cells were not blocked from forming neurites when exposed to 10 microM retinoic acid. Even more extensive treatment of LAN-5 cells with 20 microM D-PDMP (6 day pretreatment followed by 6 days together with 10 microM retinoic acid) still did not block the retinoic acid-induced neurite formation. An element of neuroblastoma tumor cell differentiation, neurite formation, is therefore dependent neither on an intact cellular ganglioside complement nor on new ganglioside synthesis.
R Li; S Ladisch
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  272     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1997 Jan 
Date Detail:
Created Date:  1997-02-12     Completed Date:  1997-02-12     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1349-54     Citation Subset:  IM    
Center for Cancer and Transplantation Biology, Children's Research Institute, Washington, D.C. 20010, USA.
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MeSH Terms
Enzyme Inhibitors / pharmacology
Gangliosides / biosynthesis*
Glucosyltransferases / antagonists & inhibitors
Morpholines / pharmacology
Neurites / metabolism*
Neuroblastoma / metabolism*
Tretinoin / pharmacology*
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Gangliosides; 0/Morpholines; 302-79-4/Tretinoin; 73257-80-4/RV 538; EC 2.4.1.-/Glucosyltransferases; EC glucosyltransferase

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