Document Detail


Inhibition effect of Oncostatin M on metastatic human lung cancer cells 95-D in vitro and on murine melanoma cells B16BL6 in vivo.
MedLine Citation:
PMID:  16971773     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Oncostatin M (OSM) is a multifunctional regulator of cell growth and differentiation. It inhibits the growth of many types of tumor cells, but its role in metastasis is unknown. We studied the human OSM expressed and purified from reconstructed E. Coli on its activity of inhibiting metastasis of tumor cells by a series of assays in vitro and in vivo. Clone formation assay in soft agar was used to measure the inhibition activity of OSM on the proliferation of high metastatic human lung cancer cells 95-D. Cell attachment assay, cell migration assay and cell invasion assay were used to evaluate inhibition by OSM on 95-D cells of the adhesion ability, the migration ability, and the ability of cells to cross tissue barriers, respectively. Inhibition of OSM on secretion of MMP-2 and -9 secretion in 95-D cells was determined by Western blot. The in vivo inhibitory effect of OSM on metastasis of murine melanoma cells B16BL6 was examined in the pulmonary metastasis model. In vitro studies showed that OSM inhibited the proliferation of 95-D cells at low concentration. OSM also reduced the adhesion and invasion ability of 95-D cells and inhibited the secretion of MMP-2 and MMP-9 in OSM treated cells. In vivo results showed that OSM (20 microg/kg/d for 7 days) inhibited pulmonary metastasis at a rate of 20.7%. There were no differences in animal weights among the groups. These results suggest that OSM has the potential of being a clinical inhibitor on metastasis of some cancer cells.
Authors:
Liming Ouyang; Li Yun Shen; Ting Li; Jianwen Liu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomedical research (Tokyo, Japan)     Volume:  27     ISSN:  0388-6107     ISO Abbreviation:  Biomed. Res.     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-09-14     Completed Date:  2006-10-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8100317     Medline TA:  Biomed Res     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  197-202     Citation Subset:  IM    
Affiliation:
School of Bioengineering, State Key Laboratory of Bioreactor Engineering, Shanghai, PR China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antineoplastic Agents / pharmacology*
Cytokines / pharmacology*
Humans
Lung Neoplasms / drug therapy*,  pathology
Melanoma, Experimental / drug therapy*,  pathology
Mice
Neoplasm Metastasis
Oncostatin M
Recombinant Proteins / pharmacology
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Cytokines; 0/OSM protein, human; 0/Osm protein, mouse; 0/Recombinant Proteins; 106956-32-5/Oncostatin M

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