Document Detail


Inhibition effect of Chinese herbal medicine on transcription of hepatitis C virus structural gene in vitro.
MedLine Citation:
PMID:  15962388     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To investigate the inhibitory effect of Chinese herbal medicine on the transcription of hepatitis C virus (HCV) structural gene in Hela D cells. METHODS: Hela cell line was transfected with recombinant pBK-CMV-HCV containing HCV structural gene by Lipofectamine. RT-nested-PCR and Western blot assay were used to testify the HCV gene expression in Hela cells. The Hela cells expressing HCV structural protein were named Hela D cells. Prescriptions of Xiao chaihu Decoction (XCHD), Fufang Huangqi (FFHQ) and Bingganling (BGL) were respectively added to Hela D cells in various concentrations. Semi-quantitative RT-nested-PCR product analysis was performed according to the fluorescent density between HCV DNA band and GAPDH DNA band in gel electrophoresis after screened. RESULTS: Recombinant pBK-CMV-HCV could correctly express the HCV structural gene in Hela D cells. After co-culture of Hela D cells with three prescriptional different concentrations for 48 h respectively, the transcription of HCV gene decreased with increasing of the concentration of each prescription. The lightness ratio of HCV product bands to GAPDH product bands was 0.24, 0.10 and 0.12 in Hela D cells incubated with 0.1 g/mL of XCHD, FFHQ and BGL respectively and the lightness ratio HCV product bands to GAPDH product bands was 0.75, 0.67 and 0.61 respectively in the control cells. CONCLUSION: The prescriptions of XCHD, FFHQ and BGL partly inhibit the transcription of HCV structural gene in Hela D cells.
Authors:
Jun Dou; Qian Chen; Jing Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  11     ISSN:  1007-9327     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-06-17     Completed Date:  2005-11-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  3619-22     Citation Subset:  IM    
Affiliation:
Department of Pathogenic Biology and Immunology, Southeast University School of Basic Medical Science, Nanjing 210009, Jiangsu Province, China. njdoujun@yahoo.com.cn
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Cell Survival
DNA Primers
Drugs, Chinese Herbal / pharmacology*
Gene Expression Regulation, Viral
Hela Cells
Hepacivirus / genetics*
Humans
Transcription, Genetic*
Transfection
Viral Structural Proteins / genetics*
Chemical
Reg. No./Substance:
0/DNA Primers; 0/Drugs, Chinese Herbal; 0/Viral Structural Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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