| Inhibition of diphosphatidylglycerol synthesis by u.v. A radiations in N.C.T.C. 2544 human keratinocytes. | |
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MedLine Citation:
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PMID: 8166663 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of u.v. A radiations on phospholipid synthesis were studied in the N.C.T.C. 2544 human keratinocyte cell line, by using [14C]arachidonic acid, [14C]oleic acid or sodium [32P]orthophosphate as precursors. Cells were irradiated in Hanks' medium with 365 nm light at doses up to 19 J/cm2, and then phospholipid synthesis from the three precursors was studied. Under these conditions, only small alterations in the incorporation pattern of [14C]arachidonic into phospholipids [phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylinositol (PI)] were observed, for u.v. A irradiation doses up to 19 J/cm2. In contrast, with [14C]oleic acid as precursor, two additional spots were observed, which co-migrate with pure phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG) standards. The incorporation of [14C]oleic acid into PG and DPG was decreased in a dose-dependent manner after u.v. A exposure, with about 50% and 75% decreases at 9.5 J/cm2 and 19 J/cm2 respectively. As for arachidonic acid incorporation, no significant differences in the synthesis of the major phospholipids (PC, PE, PI) were noted upon u.v. A exposure. The dramatic and selective decrease in PG and DPG syntheses was confirmed with [32P]orthophosphate as precursor. As DPG is a specific component of the mitochondrial inner membrane, it appears that one of the early kinds of damage induced by u.v. A irradiation could be the impairment of mitochondrial functions. |
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Authors:
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M Djavaheri-Mergny; L Mora; C Mazière; M Auclair; R Santus; L Dubertret; J C Mazière |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Biochemical journal Volume: 299 ( Pt 1) ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1994 Apr |
Date Detail:
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Created Date: 1994-05-20 Completed Date: 1994-05-20 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 85-90 Citation Subset: IM |
Affiliation:
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Laboratoire de Physico-Chimie de l'Adaptation Biologique, INSERM U312, Muséum National d'Histoire Naturelle de Paris, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cardiolipins
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biosynthesis* Cell Line Humans Keratinocytes / metabolism, radiation effects* Kinetics Thiobarbituric Acid Reactive Substances / metabolism Ultraviolet Rays* |
| Chemical | |
Reg. No./Substance:
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0/Cardiolipins; 0/Thiobarbituric Acid Reactive Substances |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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