| Inhibition of connexin 43 expression and function in cultured rat dental pulp cells by antisense oligonucleotide. | |
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MedLine Citation:
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PMID: 17450382 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Connexins are gap-junction proteins forming hexameric structures in the plasma membranes of adjacent cells, thereby creating intercellular channels. Connexin 43 (CX43) is expressed in pulp tissue. However, its function in dental pulp tissue has yet to be fully investigated. We have employed antisense oligonucleotides (AS) against rat CX43 to study the role of CX43 in dental pulp cells. Cultured dental pulp cells were treated with AS or sense (S) oligonucleotides. The number of cells in the AS-treated groups was approximately 1.3-fold that in the S-treated controls. Growth rates were significantly different between the AS- and S-treated groups at 48 h (P < 0.01). An alkaline phosphatase assay revealed that AS-treated pulp cells dramatically decreased at 48 h after AS incorporation, whereas S-treated pulp cells showed no marked changes. Western blot analysis revealed that heat-shock protein 25 was highly expressed in S-treated cells but was only weakly expressed in AS-treated cells at 48 h. Furthermore, AS-treated cells highly expressed CX45, whereas S-treated cells exhibited high expression of CX32. These results suggest that CX43 is involved in cell growth, mineralization, and differentiation to odontoblasts in rat pulp cells, and that CX43 plays the opposite role to that of CX45. |
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Authors:
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Chul-Kyun Chung; Takashi Muramatsu; Tomoko Uekusa; Hodaka Sasaki; Masaki Shimono |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-04-21 |
Journal Detail:
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Title: Cell and tissue research Volume: 329 ISSN: 0302-766X ISO Abbreviation: Cell Tissue Res. Publication Date: 2007 Aug |
Date Detail:
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Created Date: 2007-07-02 Completed Date: 2007-12-13 Revised Date: 2010-05-26 |
Medline Journal Info:
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Nlm Unique ID: 0417625 Medline TA: Cell Tissue Res Country: Germany |
Other Details:
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Languages: eng Pagination: 295-300 Citation Subset: IM |
Affiliation:
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Department of Pathology, Tokyo Dental College, 1-2-2, Masago, Mihama-ku, Chiba, 261-8502, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alkaline Phosphatase
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metabolism Animals Cell Proliferation Cells, Cultured Connexin 43 / biosynthesis*, genetics, physiology Connexins / biosynthesis Dental Pulp / cytology, metabolism* HSP27 Heat-Shock Proteins Heat-Shock Proteins / biosynthesis Incisor / cytology Neoplasm Proteins / biosynthesis Oligonucleotides / pharmacology Oligonucleotides, Antisense / pharmacology* Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/Connexin 43; 0/Connexins; 0/HSP27 Heat-Shock Proteins; 0/Heat-Shock Proteins; 0/Hspb1 protein, rat; 0/Neoplasm Proteins; 0/Oligonucleotides; 0/Oligonucleotides, Antisense; 0/connexin 32; 0/connexin 45; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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