Document Detail


Inhibition of naïve class I-restricted T cells by altered peptide ligands.
MedLine Citation:
PMID:  10457198     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Amino acid variants of an antigenic peptide or altered peptide ligands have previously been investigated with CD4+ and CD8+ T cells. However, for CD8+ T cells, only clones (which are continually restimulated in vitro) have been assessed. Using TCR transgenic mice specific for a class I Kb-restricted OVA peptide (OVAp; OT-I mice) as a source of naïve CD8+ T cells, single amino acid variants of the OVAp were analysed in vitro for their ability to antagonize the proliferative and cytotoxic function of naïve OT-I cells. Peptides with substitutions at TCR contact residues were found to be the most potent antagonists of OT-I cell function. Those peptides that inhibited activation of cells to proliferate also inhibited activation of cells to become killers. Inhibition was inversely correlated with interferon (IFN)-gamma production. It was found that levels of antagonist peptide required for inhibition were higher than that described for T cell clones, presumably due to affinity differences.
Authors:
C Koniaras; F R Carbone; W R Heath; A M Lew
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Immunology and cell biology     Volume:  77     ISSN:  0818-9641     ISO Abbreviation:  Immunol. Cell Biol.     Publication Date:  1999 Aug 
Date Detail:
Created Date:  1999-09-23     Completed Date:  1999-09-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8706300     Medline TA:  Immunol Cell Biol     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  318-23     Citation Subset:  IM; X    
Affiliation:
The Walter and Eliza Hall Institute, Monash Medical School, Melbourne, Victoria, Australia.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
CD8-Positive T-Lymphocytes / drug effects*,  immunology*
Cytokines / secretion
Cytotoxicity, Immunologic / drug effects
Histocompatibility Antigens Class I
Ligands
Lymphocyte Activation / drug effects
Mice
Mice, Transgenic
Ovalbumin / chemistry,  genetics,  immunology
Peptides / chemistry,  immunology*,  pharmacology*
Receptors, Antigen, T-Cell, alpha-beta / genetics
Chemical
Reg. No./Substance:
0/Cytokines; 0/Histocompatibility Antigens Class I; 0/Ligands; 0/Peptides; 0/Receptors, Antigen, T-Cell, alpha-beta; 9006-59-1/Ovalbumin

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