| Inhibition of chemotaxis Ng-monomethyl-L-arginine: a role for cyclic GMP. | |
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MedLine Citation:
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PMID: 2553158 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The metabolism of L-arginine to nitric oxide (NO) has been shown to be important for the effector functions of many cell types, including polymorphonuclear (PMN) leukocytes. Its effect appears to be mediated at least in part by NO stimulation of soluble guanylate cyclase. We evaluated the role of this pathway in two PMN effector functions: cell movement and microbial killing, using the competitive inhibitor of L-arginine conversion to NO, NG-monomethyl-L-arginine (NMA). We also evaluated the effect of additional L-arginine and dibutyryl cyclic guanosine monophosphate (cGMP) on any NMA-associated changes. Human peripheral blood neutrophils were used and the cells were incubated with and without NMA. Chemotaxis was evaluated using a 48-well micro-Boyden chamber. Microbial killing was evaluated using S aureus strains D2C and 502A. These studies demonstrated that chemotaxis to formyl-methionyl-leucyl-phenylalanine was markedly inhibited in NMA-treated cells. This inhibition could be overcome if L-arginine or dibutyryl cGMP were added with the NMA. In contrast, microbial killing of S aureus was unaffected by NMA. These observations support the hypothesis that the L-arginine metabolism to NO and its effect on the cGMP level may be important for the dynamic changes required for neutrophil chemotaxis. |
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Authors:
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S S Kaplan; T Billiar; R D Curran; U E Zdziarski; R L Simmons; R E Basford |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Blood Volume: 74 ISSN: 0006-4971 ISO Abbreviation: Blood Publication Date: 1989 Nov |
Date Detail:
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Created Date: 1989-12-08 Completed Date: 1989-12-08 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7603509 Medline TA: Blood Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1885-7 Citation Subset: AIM; IM |
Affiliation:
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Department of Pathology, University of Pittsburgh School of Medicine, PA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Arginine
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pharmacology Blood Bactericidal Activity / drug effects Chemotaxis, Leukocyte / drug effects* Cyclic GMP / physiology* Dibutyryl Cyclic GMP / pharmacology Humans N-Formylmethionine Leucyl-Phenylalanine / pharmacology Nitric Oxide / metabolism omega-N-Methylarginine |
| Grant Support | |
ID/Acronym/Agency:
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AI-14032/AI/NIAID NIH HHS; GM-37753/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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10102-43-9/Nitric Oxide; 17035-90-4/omega-N-Methylarginine; 32266-35-6/Dibutyryl Cyclic GMP; 59880-97-6/N-Formylmethionine Leucyl-Phenylalanine; 74-79-3/Arginine; 7665-99-8/Cyclic GMP |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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