Document Detail


Inhibition of cellular DNA synthesis and lack of antileukemic activity by non-photoactivated hematoporphyrin derivative.
MedLine Citation:
PMID:  7281237     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It has been reported that cytocidal activity of light-activated hematoporphyrin (HPD) within the cells might be exploited in the therapy of experimental and human cancer. As part of a project from this laboratory aimed to study some major biologic features of HPD, it was found that [3H]thymidine incorporation in tumor cells was highly inhibited as a consequence of HPD treatment. HPD-mediated inhibition, obtained by a treatment either in vitro or in vivo, was long lasting and independent of light activation. Cellular DNA synthesis was inhibited by non toxic doses of HPD which were not influential either cell viability or cell oncogenicity. In preliminary studies, HPD-treated cells accumulated in the G1 phase of the cell cycle as detected by cytofluorometric analysis. This finding is in keeping with a likely inhibition exerted in late G1 or at the beginning of the S phase of cell the cycle and might exclude a direct damage of the DNA synthetic machinery. Definitive loss of cell viability and cellular DNA inhibition was obtained immediately after the exposure of HPD-treated cells to He-Ne laser light. HPD-mediated cell lysis was dose dependent and in the other of magnitude of cytocidal doses in different cell systems. HPD antileukemic activity or HPD interactions with chemotherapeutic drugs was ruled out in L1210 leukemic mice.
Authors:
P Franco; S Morelli; F Sarra; A Nicolin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Tumori     Volume:  67     ISSN:  0300-8916     ISO Abbreviation:  Tumori     Publication Date:    1981 May-Jun
Date Detail:
Created Date:  1981-11-18     Completed Date:  1981-11-18     Revised Date:  2008-12-12    
Medline Journal Info:
Nlm Unique ID:  0111356     Medline TA:  Tumori     Country:  ITALY    
Other Details:
Languages:  eng     Pagination:  183-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antineoplastic Agents / therapeutic use
Cell Cycle
Cell Survival
DNA, Neoplasm / biosynthesis*
Female
Hematoporphyrins / pharmacology*
Lasers
Leukemia L1210 / drug therapy*
Light
Male
Mice
Mice, Inbred DBA
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/DNA, Neoplasm; 0/Hematoporphyrins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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