Document Detail


Inhibition of cell-surface neutral protease of Ehrlich ascites tumor cells by potassium p-(3,3-dimethyl-1-triazeno) benzoate.
MedLine Citation:
PMID:  3902023     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ehrlich ascites tumour (EAT) cells possess a trypsin-like neutral protease on the cell surface. The antimetastatic triazene drug potassium p-(3,3-dimethyl-1-triazeno) benzoate (DM-COOK) inhibits this neutral protease, and also trypsin. Incubation of EAT cells with human erythrocytes (ratio of 1 to 5) at 37 degrees C for 18 h caused haemolysis of 28.8% of the erythrocytes. Addition of millimolar concentrations of DM-COOK to a fixed quantity (2.5 X 10(8)) of EAT cells caused a dose-related inhibition of haemolysis. DM-COOK was strongly bound to EAT cells and could not be removed by repeated washing.
Authors:
F S Steven; B S Brown; T P Hulley; S Itzhaki; T Giraldi; L Lassiani
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Biochemistry international     Volume:  11     ISSN:  0158-5231     ISO Abbreviation:  Biochem. Int.     Publication Date:  1985 Aug 
Date Detail:
Created Date:  1985-11-05     Completed Date:  1985-11-05     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8100311     Medline TA:  Biochem Int     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  153-60     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aminopeptidases / antagonists & inhibitors
Animals
Antineoplastic Agents / pharmacology
Carcinoma, Ehrlich Tumor / enzymology*
Cathepsin H
Cathepsins*
Cell Membrane / enzymology
Cysteine Endopeptidases*
Hemolysis / drug effects
Humans
Mice
Neoplasm Metastasis
Neprilysin
Protease Inhibitors*
Triazenes / pharmacology*
Trypsin Inhibitors
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Protease Inhibitors; 0/Triazenes; 0/Trypsin Inhibitors; 7203-91-0/1-(4-carboxyphenyl)-3,3-dimethyltriazene; EC 3.4.-/Cathepsins; EC 3.4.11.-/Aminopeptidases; EC 3.4.22.-/Cysteine Endopeptidases; EC 3.4.22.16/CTSH protein, human; EC 3.4.22.16/Cathepsin H; EC 3.4.22.16/Ctsh protein, mouse; EC 3.4.24.11/Neprilysin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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