Document Detail


Inhibition of cell proliferation: a mechanism likely to mediate the prevention of neuronal cell death by melatonin.
MedLine Citation:
PMID:  9694399     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In a previous work we demonstrated that melatonin is able to prevent apoptosis induced by low doses of 6-hydroxydopamine (6-OHDA) in undifferentiated and neuronal PC12 cells. We also reported how this neurohormone was able to prevent the decrease in the mRNA for antioxidant enzymes caused by 6-OHDA. Although the antioxidant capability of melatonin seems to be clearly implicated in its antiapoptotic activity, literature suggests that its antiproliferative property could also be involved in its prevention of apoptosis. In the present work we demonstrated that melatonin is able to inhibit cell proliferation in undifferentiated PC12 cells, decreasing cell number and the total amount of DNA, and the mRNA for the histone H4, which are known to increase during DNA synthesis. Melatonin does not decrease the number of cells in nonproliferating PC12 cells, indicating that it does not cause cell death. Additionally, we demonstrate that other inhibitors of cell proliferation, as well as other antioxidants, are able to mimic the antiapoptotic effect of melatonin. This is interpreted to mean that melatonin acts by both mechanisms to inhibit apoptosis caused by 6-OHDA and the findings support the hypothesis of a relationship between oxidative stress and regulation of the cell cycle.
Authors:
J C Mayo; R M Sainz; H Uría; I Antolín; M M Estéban; Rodríguez
Related Documents :
11180409 - Cell-cycle arrest in trka-expressing nih3t3 cells involves nitric oxide synthase.
12843659 - Effect of neuronal pc12 cells on the functional properties of intestinal epithelial cac...
7509449 - Expression of the v-crk oncogene product in pc12 cells results in rapid differentiation...
7881099 - Vitamin d receptors in breast cancer cells.
8824899 - Gene expression of nor-1, a neuron-derived orphan receptor, is inducible in neuronal an...
20577899 - Protective effects of paeoniflorin against glutamate-induced neurotoxicity in pc12 cell...
18575829 - Extracellular atp and cancer: an overview with special reference to p2 purinergic recep...
12787309 - Immunohistochemical demonstration of 14-3-3 sigma protein in normal human tissues and l...
15689959 - Acidosis causes endoplasmic reticulum stress and caspase-12-mediated astrocyte death.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of pineal research     Volume:  25     ISSN:  0742-3098     ISO Abbreviation:  J. Pineal Res.     Publication Date:  1998 Aug 
Date Detail:
Created Date:  1998-09-18     Completed Date:  1998-09-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8504412     Medline TA:  J Pineal Res     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  12-8     Citation Subset:  IM    
Affiliation:
Departamento de Morfología y Biología Celular, Universidad de Oviedo, Facultad de Medicina, Spain.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Antineoplastic Agents / pharmacology
Antioxidants / pharmacology*
Blotting, Northern
Cell Count
Cell Cycle / drug effects
Cell Differentiation
Cell Division / drug effects*
Cell Survival / drug effects*
DNA / metabolism
DNA Fragmentation
Histones / genetics,  metabolism
Male
Melatonin / pharmacology*
Oxidopamine / pharmacology
PC12 Cells / cytology*,  metabolism
RNA, Messenger / metabolism
Rats
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Antioxidants; 0/Histones; 0/RNA, Messenger; 1199-18-4/Oxidopamine; 73-31-4/Melatonin; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  An essential role for ovarian inhibin in pineal gland-mediated anestrus in Syrian hamsters.
Next Document:  Direct neuronal projection from the dorsal raphe nucleus to the pineal complex of the rat: a Phaseol...