Document Detail

Inhibition of cell proliferation: a mechanism likely to mediate the prevention of neuronal cell death by melatonin.
MedLine Citation:
PMID:  9694399     Owner:  NLM     Status:  MEDLINE    
In a previous work we demonstrated that melatonin is able to prevent apoptosis induced by low doses of 6-hydroxydopamine (6-OHDA) in undifferentiated and neuronal PC12 cells. We also reported how this neurohormone was able to prevent the decrease in the mRNA for antioxidant enzymes caused by 6-OHDA. Although the antioxidant capability of melatonin seems to be clearly implicated in its antiapoptotic activity, literature suggests that its antiproliferative property could also be involved in its prevention of apoptosis. In the present work we demonstrated that melatonin is able to inhibit cell proliferation in undifferentiated PC12 cells, decreasing cell number and the total amount of DNA, and the mRNA for the histone H4, which are known to increase during DNA synthesis. Melatonin does not decrease the number of cells in nonproliferating PC12 cells, indicating that it does not cause cell death. Additionally, we demonstrate that other inhibitors of cell proliferation, as well as other antioxidants, are able to mimic the antiapoptotic effect of melatonin. This is interpreted to mean that melatonin acts by both mechanisms to inhibit apoptosis caused by 6-OHDA and the findings support the hypothesis of a relationship between oxidative stress and regulation of the cell cycle.
J C Mayo; R M Sainz; H Uría; I Antolín; M M Estéban; Rodríguez
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of pineal research     Volume:  25     ISSN:  0742-3098     ISO Abbreviation:  J. Pineal Res.     Publication Date:  1998 Aug 
Date Detail:
Created Date:  1998-09-18     Completed Date:  1998-09-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8504412     Medline TA:  J Pineal Res     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  12-8     Citation Subset:  IM    
Departamento de Morfología y Biología Celular, Universidad de Oviedo, Facultad de Medicina, Spain.
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MeSH Terms
Antineoplastic Agents / pharmacology
Antioxidants / pharmacology*
Blotting, Northern
Cell Count
Cell Cycle / drug effects
Cell Differentiation
Cell Division / drug effects*
Cell Survival / drug effects*
DNA / metabolism
DNA Fragmentation
Histones / genetics,  metabolism
Melatonin / pharmacology*
Oxidopamine / pharmacology
PC12 Cells / cytology*,  metabolism
RNA, Messenger / metabolism
Reg. No./Substance:
0/Antineoplastic Agents; 0/Antioxidants; 0/Histones; 0/RNA, Messenger; 1199-18-4/Oxidopamine; 73-31-4/Melatonin; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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