Document Detail

Inhibition of cell proliferation and induction of apoptosis by genistein in experimental hepatocellular carcinoma.
MedLine Citation:
PMID:  17006617     Owner:  NLM     Status:  MEDLINE    
Hepatocellular carcinoma (HCC) is the leading cause of cancer related deaths in the world, with increasing incidence in many developed countries. Epidemiological data suggest that consumption of soy products may be associated with a decreased risk of cancer. We investigate the effects of genistein on cell proliferation, apoptosis and caspase-3 in DEN induced (200 mg/kg body weight; by single intraperitoneal injection) and Phenobarbital promoted (0.05% through drinking water for 14 successive weeks) cancer-bearing rats. Immunohistochemistry was employed to detect cell proliferating markers proliferating cell nuclear antigen (PCNA), DNA fragmentation was determined by agarose gel electrophoresis and terminal deoxynucleatide transferase dUTP nick labeling (TUNEL) staining and caspase by enzyme-linked immunosorbent assay. We found inhibition of cell proliferation, induction of apoptosis and activation of caspase-3 in genistein treated animals. From these results, we conclude that genistein inhibit cell proliferation, induced apoptosis. This activation of caspsase-3 in genistein treated liver cancer bearing animals correlated well with its apoptosis inducing effect.
Dechen Chodon; S Mumtaz Banu; R Padmavathi; D Sakthisekaran
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-09-28
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  297     ISSN:  0300-8177     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-15     Completed Date:  2007-06-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  73-80     Citation Subset:  IM    
Department of Medical Biochemistry, Dr. ALM Post Graduate Institute of Basic, Medical Science, University of Madras, Taramani Campus, Chennai 600 113, India.
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MeSH Terms
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects*
Caspase 3 / metabolism
Cell Proliferation / drug effects
DNA Fragmentation / drug effects
Electrophoresis, Agar Gel
Genistein / pharmacology*
In Situ Nick-End Labeling
Liver / drug effects,  enzymology,  pathology
Liver Neoplasms, Experimental / enzymology,  pathology*
Proliferating Cell Nuclear Antigen / metabolism
Rats, Wistar
Reg. No./Substance:
0/Antineoplastic Agents; 0/Proliferating Cell Nuclear Antigen; 446-72-0/Genistein; EC 3.4.22.-/Caspase 3

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