Document Detail


Inhibition of cell proliferation by glycerol.
MedLine Citation:
PMID:  1901931     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of glycerol on proliferation of BHK, CHO, HBL, MCF-7, and human glioma cells was studied. Cell proliferation was significantly decreased in all the cell lines at glycerol concentrations of 2-4% in the culture medium. The inhibition was dose-dependent, complete suppression of proliferation occurring at a glycerol concentration of 4% for the MCF-7 cell line and 6-8% for the BHK, CHO and human glioma cells. Studies on [3H]thymidine incorporation correlate with the effect on cell proliferation. The viability of the cells was not significantly affected until higher concentrations of glycerol (12% +) were present. Recovery studies with BHK cells indicated that replacement of the glycerol medium with glycerol-free medium resulted in full recovery following exposure to 4% glycerol and only partial recovery (65%) of proliferation rate following exposure to 10-12% glycerol. It is concluded that glycerol, a substance that is normally present in tissues, can serve as a potent inhibitor of cell proliferation.
Authors:
J P Wiebe; C J Dinsdale
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Life sciences     Volume:  48     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  1991  
Date Detail:
Created Date:  1991-05-21     Completed Date:  1991-05-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1511-7     Citation Subset:  IM    
Affiliation:
Department of Zoology, University of Western Ontario, London, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle
Cell Division / drug effects*
Cell Line
Cricetinae
Culture Media
Glycerol / pharmacology*
Haplorhini
Humans
Thymidine / metabolism
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Culture Media; 50-89-5/Thymidine; 56-81-5/Glycerol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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