| Inhibition of cell proliferation and arrest of cell cycle progression by blocking chloride channels in human laryngeal cancer cell line Hep-2. | |
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MedLine Citation:
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PMID: 19309225 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Chloride channel (ClC) is involved in normal physiological processes and pathology of various diseases. Although it is recognized that blockade of ClC inhibits the cell proliferation, it is not well understood the potential function of ClC in laryngeal cancer. In this study, we investigated the effect of the ClC inhibitor on cell proliferation, cell cycle progression in human laryngeal cancer cell line Hep-2, as well as the effect on the phosphorylation levels of ERK1/2 and AKT1. In this study crystal violet method was used to study the effect of the ClC inhibitor, 5-nitro-2-(3-phenylpropylamino) benzoic acid, NPPB, on Hep-2 cell proliferation. The impaction of the inhibitor on the cell cycle distribution was investigated by the flow cytometry (FCM). Western blot was performed to measure the phosphorylation levels of ERK1/2 and AKT1. Our data indicated ClC played an important role in Hep-2 cell proliferation and cell cycle. NPPB inhibited Hep-2 cell proliferation when compared with the controls. Blockade of ClC arrested cell cycle progression and suppressed the phosphorylation of ERK1/2 and AKT1 in Hep-2 cells by inhibition of cell proliferation by CIC inhibitor (NPPB) could be through arresting cell cycle progression, which is probably by suppressing phosphorylation of ERK1/2 and AKT1. |
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Authors:
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W F Yu; Y L Zhao; K Wang; M M Dong |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Neoplasma Volume: 56 ISSN: 0028-2685 ISO Abbreviation: Neoplasma Publication Date: 2009 |
Date Detail:
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Created Date: 2009-03-24 Completed Date: 2009-07-15 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0377266 Medline TA: Neoplasma Country: Slovakia |
Other Details:
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Languages: eng Pagination: 224-9 Citation Subset: IM |
Affiliation:
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Department of Otolaryngology, the first Affiliated Hospital of Zhengzhou University, Zhengzhou, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Cycle
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drug effects Cell Line, Tumor Cell Proliferation / drug effects Chloride Channels / antagonists & inhibitors*, physiology Dose-Response Relationship, Drug Extracellular Signal-Regulated MAP Kinases / metabolism Humans Laryngeal Neoplasms / drug therapy, pathology* Nitrobenzoates / pharmacology* Phosphorylation Proto-Oncogene Proteins c-akt / metabolism Signal Transduction |
| Chemical | |
Reg. No./Substance:
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0/Chloride Channels; 0/Nitrobenzoates; 107254-86-4/5-nitro-2-(3-phenylpropylamino)benzoic acid; EC 2.7.1.37/AKT1 protein, human; EC 2.7.11.1/Proto-Oncogene Proteins c-akt; EC 2.7.11.24/Extracellular Signal-Regulated MAP Kinases |
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