Document Detail


Inhibition of cell growth and induction of G1-phase cell cycle arrest in hepatoma cells by steroid extract from Meretrix meretrix.
MedLine Citation:
PMID:  16458116     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, we report that the steroid extract 5alpha, 8alpha-epidioxycholest-6-ene-3beta-ol (MME) from Meretrix meretrix has the ability to inhibit growth of hepatoma cells and to induce G1-phase cell cycle arrest in two human hepatoma cell lines, HepG2 and Hep3B. HepG2 cells were more sensitive than Hep3B to MME. The extract markedly up-regulated the expression of p53 and p21WAF1/CIP1 in HepG2, suggesting that MME-induced G1 phase cell cycle arrest in HepG2 might be p53-dependent. Therefore, the up-regulation of p27KIP1and p16INK4A in both cell lines indicates that a p53-independent pathway might be involved in the mechanism of MME inducing cell cycle arrest. In conclusion, MME induces G1 phase cell cycle arrest via both p53-dependent and p53-independent pathways.
Authors:
Ting-He Wu; Ruo-Lin Yang; Li-Ping Xie; Hong-Zhong Wang; Lei Chen; Shuyi Zhang; Yong Zhao; Rong-Qing Zhang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cancer letters     Volume:  232     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-02-06     Completed Date:  2006-04-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  199-205     Citation Subset:  IM    
Affiliation:
Laboratory of Marine Biotechnology, Department of Biological Science and Biotechnology, Tsinghua University, Beijing 100084, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD95 / analysis
Bivalvia / chemistry*
Carcinoma, Hepatocellular / chemistry,  drug therapy*,  pathology
Cell Line, Tumor
Cell Proliferation / drug effects
Cyclin-Dependent Kinase Inhibitor p16 / analysis
Cyclin-Dependent Kinase Inhibitor p21 / analysis
Cyclin-Dependent Kinase Inhibitor p27 / analysis
G1 Phase / drug effects*
Humans
Intracellular Signaling Peptides and Proteins / analysis
Liver Neoplasms / chemistry,  drug therapy*,  pathology
Steroids / pharmacology*
Tumor Suppressor Protein p53 / analysis
Chemical
Reg. No./Substance:
0/Antigens, CD95; 0/CDKN1A protein, human; 0/CDKN1B protein, human; 0/Cyclin-Dependent Kinase Inhibitor p16; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Intracellular Signaling Peptides and Proteins; 0/Steroids; 0/Tumor Suppressor Protein p53; 147604-94-2/Cyclin-Dependent Kinase Inhibitor p27

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