| Inhibition of catalase activity as an early response of Arabidopsis thaliana cultured cells to the phytotoxin fusicoccin. | |
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MedLine Citation:
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PMID: 18039736 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In Arabidopsis thaliana cells, fusicoccin (FC) treatment induced an early and marked increase in the extracellular H(2)O(2) level. It also increased the huge hypo-osmotic stress-induced oxidative wave and, in addition, prevented the H(2)O(2) peak drop. These effects were apparently not linked to changes in either cytoplasmic pH or cytoplasmic free calcium concentration, since they occurred independently of the activity state of the plasma membrane (PM) H(+)-ATPase and neither influx nor efflux of (45)Ca(2+) was modified by FC. In the presence of diphenylene iodonium (DPI), inhibiting the PM NADPH oxidase presumably responsible for reactive oxygen species (ROS) production, no apoplastic H(2)O(2) development was detected either with or without FC. However, no increase in DPI-sensitive ferricyanide reduction, but rather a gradual decrease, occurred with FC. These results suggested that the H(2)O(2) increase observed with FC was not due to a overproduction of ROS but, more probably, to a reduced capability of FC-treated cells to degrade the H(2)O(2) formed. This view, at first supported by the finding that FC-treated cells failed to break down exogenously supplied H(2)O(2), was clearly confirmed by a series of measurements on exogenous catalase activity, tested in cell-free media of FC-treated samples. This assay, in fact, allowed ascertainment and partial characterization of an as yet unidentified factor increasingly accumulating in the incubation medium of FC-treated cells, behaving as a non-competitive catalase inhibitor and able to reduce markedly the cell's capability for H(2)O(2) scavenging. |
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Authors:
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Nicoletta Beffagna; Irene Lutzu |
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Publication Detail:
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Type: Journal Article Date: 2007-11-26 |
Journal Detail:
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Title: Journal of experimental botany Volume: 58 ISSN: 1460-2431 ISO Abbreviation: J. Exp. Bot. Publication Date: 2007 |
Date Detail:
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Created Date: 2008-01-09 Completed Date: 2008-04-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9882906 Medline TA: J Exp Bot Country: England |
Other Details:
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Languages: eng Pagination: 4183-94 Citation Subset: IM |
Affiliation:
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Istituto di Biofisica del CNR-Sezione di Milano, Dipartimento di Biologia, Università degli Studi di Milano, Via Celoria 26, 20133 Milano, Italy. nicoletta.beffagna@unimi.it |
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| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Infective Agents
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pharmacology Arabidopsis / drug effects* Butyric Acids / pharmacology Calcium / metabolism Catalase / antagonists & inhibitors* Cell Membrane / metabolism* Cells, Cultured Cytoplasm / metabolism Drug Synergism Eosine Yellowish-(YS) / pharmacology Ferricyanides / metabolism Fluorescent Dyes / pharmacology Glycosides / pharmacology* Hydrogen Peroxide / metabolism* Hydrogen-Ion Concentration Oxidation-Reduction Salicylic Acid / pharmacology Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Anti-Infective Agents; 0/Butyric Acids; 0/Ferricyanides; 0/Fluorescent Dyes; 0/Glycosides; 13408-62-3/hexacyanoferrate III; 17372-87-1/Eosine Yellowish-(YS); 20108-30-9/fusicoccin; 69-72-7/Salicylic Acid; 7440-70-2/Calcium; 7722-84-1/Hydrogen Peroxide; 79-31-2/isobutyric acid; EC 1.11.1.6/Catalase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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