| Inhibition of cardiolipin biosynthesis in the hypoxic rat heart. | |
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MedLine Citation:
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PMID: 7651078 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cardiolipin is the principal polyglycerophospholipid in the heart. The effect of hypoxia on cardiolipin biosynthesis was investigated in isolated rat hearts perfused in the Langendorff mode. Hearts were pulsed-labeled for 60 min with 0.1 mM [1,(3)-3H]glycerol in Krebs Henseleit buffer saturated with either 95% O2/5% CO2 (control) or 95% N2/5% CO2 (hypoxic). Radioactivity incorporated into phosphatidylglycerol and cardiolipin were reduced 88% (P < .05) and 79% (P < .05), respectively, in hypoxic hearts compared to controls. In other experiments, hearts were pulse-labeled for 15 min with 1.4 mM [32P]Pi in Krebs Henseleit buffer saturated with 95% O2/5% CO2 and subsequently perfused for 60 min under control or hypoxic conditions. The radioactivity incorporated into CDP-1,2-diacyl-sn-glycerol, phosphatidylglycerol, and cardiolipin were reduced 61% (P < .05), 71% (P < .05), and 70% (P < .05), respectively, in the hypoxic hearts compared to controls, indicating a decreased formation of CDP-1,2-diacyl-sn-glycerol in the hypoxic heart. The activities of the enzymes involved in cardiolipin biosynthesis and the cardiac pool sizes of cardiolipin, phosphatidylglycerol, and CDP-1,2-diacyl-1,2-diacyl-sn-glycerol were unaltered between hypoxic and control hearts. In contrast, cardiac adenosine-5'-triphosphate and CPT levels were decreased 94% (P < .05) and 92% (P < .05), respectively, in hypoxic hearts compared to controls. We postulate that the biosynthesis of the cardiac polyglycerophospholipid cardiolipin may be inhibited by a decreased adenosine-5'-triphosphate and cytidine-5'-triphosphate level in the heart. |
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Authors:
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P Cheng; G M Hatch |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Lipids Volume: 30 ISSN: 0024-4201 ISO Abbreviation: Lipids Publication Date: 1995 Jun |
Date Detail:
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Created Date: 1995-09-26 Completed Date: 1995-09-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 513-9 Citation Subset: IM |
Affiliation:
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Department of Internal Medicine, University of Manitoba, Winnipeg, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism Animals Anoxia / metabolism* Cardiolipins / biosynthesis* Cytidine Diphosphate Diglycerides / metabolism Cytidine Triphosphate / metabolism Male Myocardium / metabolism* Nucleotidyltransferases / metabolism Phosphatidylglycerols / metabolism Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/Cardiolipins; 0/Cytidine Diphosphate Diglycerides; 0/Phosphatidylglycerols; 56-65-5/Adenosine Triphosphate; 65-47-4/Cytidine Triphosphate; EC 2.7.7.-/Nucleotidyltransferases; EC 2.7.7.41/phosphatidate cytidylyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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