Document Detail


Inhibition of camel lens zeta-crystallin/NADPH:quinone oxidoreductase activity by chloranilic acid.
MedLine Citation:
PMID:  9063582     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Camel lens zeta-crystallin/NADPH:quinone oxidoreductase activity was inhibited by chloranilic acid (2,5-dichloro-3,6-dihydroxy-1,4-benzoquinone) with NADPH as an electron donor and 9,10-phenanthrenequinone (PQ) as an electron acceptor in a time-independent but concentration dependent manner. The IC50 of chloranilic acid was 1 microM. The inhibition was noncompetitive with respect to both NADPH and PQ as deduced by Lineweaver-Burk plots. The estimated inhibition constant (Ki) was 0.8 microM for both NADPH and PQ. Examination of other benzoquinones suggested that the presence of -OH and -Cl on benzoquinone was essential for the inhibition.
Authors:
A A al-Hamidi; A S Riskuwa; S D Ali
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemistry and molecular biology international     Volume:  41     ISSN:  1039-9712     ISO Abbreviation:  Biochem. Mol. Biol. Int.     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-06-11     Completed Date:  1997-06-11     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9306673     Medline TA:  Biochem Mol Biol Int     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  415-21     Citation Subset:  IM    
Affiliation:
Department of Science, King Khalid Military Academy, Riyadh, Kingdom of Saudi Arabia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Benzoquinones / pharmacology*
Camels
Chloranil / pharmacology
Crystallins / antagonists & inhibitors*
Kinetics
NAD(P)H Dehydrogenase (Quinone) / antagonists & inhibitors*
Chemical
Reg. No./Substance:
0/Benzoquinones; 0/Crystallins; 118-75-2/Chloranil; 18905-34-5/tetrahydroxy-1,4-benzoquinone; 87-88-7/chloranilic acid; EC 1.6.5.2/NAD(P)H Dehydrogenase (Quinone)

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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