| Inhibition by selected food components of human and porcine intestinal pteroylpolyglutamate hydrolase activity. | |
| | |
MedLine Citation:
|
PMID: 2296933 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Studies were conducted to determine the in vitro effect of selected food components on activity of the brush border membrane pteroylpolyglutamate hydrolase (folate conjugase) of porcine and human intestine. Foods differed widely in their effects although the pattern of the effects on both porcine and human enzymes was similar. Extracts of legumes, tomatoes, and orange juice consistently inhibited the conjugase activity. Citrate was also inhibitory to some extent. In contrast, extracts of cereal grain flours, whole egg, milk, cabbage, cauliflower, and lettuce caused little inhibition. Purified phytohemagglutinins, soybean trypsin inhibitors, and bovine milk folate-binding protein had no effect on the conjugase activity at the concentrations tested. The food substances that inhibited the conjugase activity did not bind the polyglutamyl folate substrate or inhibit intestinal brush border membrane sucrase and alkaline phosphatase. These findings suggest that food composition may influence folate bioavailability by interfering with the intestinal deconjugation of dietary polyglutamyl folates. |
| | |
Authors:
|
S D Bhandari; J F Gregory |
Related Documents
:
|
17466873 - Anterior segment complications of a nutritional supplement. 3884303 - Current approaches to management of potassium deficiency. 11467993 - Reducing the need for food allergen challenges in young children: a comparison of in vi... |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
|
Title: The American journal of clinical nutrition Volume: 51 ISSN: 0002-9165 ISO Abbreviation: Am. J. Clin. Nutr. Publication Date: 1990 Jan |
Date Detail:
|
Created Date: 1990-02-22 Completed Date: 1990-02-22 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0376027 Medline TA: Am J Clin Nutr Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 87-94 Citation Subset: AIM; IM |
Affiliation:
|
Food Science and Human Nutrition Department, University of Florida, Gainesville 32611-0163. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Biological Availability Cysteine Proteinase Inhibitors* Diet* Fabaceae Fruit Humans Intestinal Absorption Intestines / enzymology*, metabolism Microvilli / enzymology Plant Extracts / pharmacology Plants, Medicinal Pteroylpolyglutamic Acids / metabolism Swine gamma-Glutamyl Hydrolase / antagonists & inhibitors* |
| Chemical | |
Reg. No./Substance:
|
0/Cysteine Proteinase Inhibitors; 0/Plant Extracts; 0/Pteroylpolyglutamic Acids; EC 3.4.19.9/gamma-Glutamyl Hydrolase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Evaluation of the deoxyuridine suppression test by using whole blood samples from folic acid-supplem...
Next Document: Effects of different calcium sources on iron absorption in postmenopausal women.