Document Detail

Inhibition by selected food components of human and porcine intestinal pteroylpolyglutamate hydrolase activity.
MedLine Citation:
PMID:  2296933     Owner:  NLM     Status:  MEDLINE    
Studies were conducted to determine the in vitro effect of selected food components on activity of the brush border membrane pteroylpolyglutamate hydrolase (folate conjugase) of porcine and human intestine. Foods differed widely in their effects although the pattern of the effects on both porcine and human enzymes was similar. Extracts of legumes, tomatoes, and orange juice consistently inhibited the conjugase activity. Citrate was also inhibitory to some extent. In contrast, extracts of cereal grain flours, whole egg, milk, cabbage, cauliflower, and lettuce caused little inhibition. Purified phytohemagglutinins, soybean trypsin inhibitors, and bovine milk folate-binding protein had no effect on the conjugase activity at the concentrations tested. The food substances that inhibited the conjugase activity did not bind the polyglutamyl folate substrate or inhibit intestinal brush border membrane sucrase and alkaline phosphatase. These findings suggest that food composition may influence folate bioavailability by interfering with the intestinal deconjugation of dietary polyglutamyl folates.
S D Bhandari; J F Gregory
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The American journal of clinical nutrition     Volume:  51     ISSN:  0002-9165     ISO Abbreviation:  Am. J. Clin. Nutr.     Publication Date:  1990 Jan 
Date Detail:
Created Date:  1990-02-22     Completed Date:  1990-02-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376027     Medline TA:  Am J Clin Nutr     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  87-94     Citation Subset:  AIM; IM    
Food Science and Human Nutrition Department, University of Florida, Gainesville 32611-0163.
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MeSH Terms
Biological Availability
Cysteine Proteinase Inhibitors*
Intestinal Absorption
Intestines / enzymology*,  metabolism
Microvilli / enzymology
Plant Extracts / pharmacology
Plants, Medicinal
Pteroylpolyglutamic Acids / metabolism
gamma-Glutamyl Hydrolase / antagonists & inhibitors*
Reg. No./Substance:
0/Cysteine Proteinase Inhibitors; 0/Plant Extracts; 0/Pteroylpolyglutamic Acids; EC Hydrolase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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