Document Detail

Inhibition by islet-activating protein, pertussis toxin, of retinoic acid-induced differentiation of human leukemic (HL-60) cells.
MedLine Citation:
PMID:  2551727     Owner:  NLM     Status:  MEDLINE    
Human promyelocytic leukemic (HL-60) cells were induced to differentiate into neutrophil- or macrophage-like cells by incubation of the cells with retinoic acid, dibutyryl cyclic AMP (Bt2cAMP) or phorbol 12-myristate 13-acetate (PMA). Differentiation was determined by an increase in the percentage of morphologically mature cells. The retinoic acid-induced differentiation of HL-60 cells was, but the Bt2cAMP- or PMA-induced one was not, inhibited by prior exposure of the cells to islet-activating protein (IAP), pertussis toxin. The IAP-induced inhibition was correlated with the toxin-catalyzed ADP-ribosylation of a membrane GTP-binding protein with a molecular mass of 40 kDa. Thus, the IAP-substrate GTP-binding protein appears to be involved in the retinoic acid-induced differentiation of HL-60 cells.
M Tohkin; T Iiri; M Ui; T Katada
Related Documents :
15161187 - Grape seed extract affects proliferation and differentiation of human intestinal caco-2...
8466527 - Geranylgeranylacetone used as an antiulcer agent is a potent inducer of differentiation...
18442987 - Cardiac differentiation in xenopus requires the cyclin-dependent kinase inhibitor, p27x...
19559667 - Development of effective isolation method of es cells for analysis of differentiation.
18643547 - Single cell mechanics: stress stiffening and kinematic hardening.
22110717 - Photoporation of biomolecules into single cells in living vertebrate embryos induced by...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEBS letters     Volume:  255     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1989 Sep 
Date Detail:
Created Date:  1989-11-09     Completed Date:  1989-11-09     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  187-90     Citation Subset:  IM    
Department of Life Science, Faculty of Science, Tokyo Institute of Technology, Yokohama, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Adenosine Diphosphate Ribose / metabolism
Cell Differentiation / drug effects
Cyclic AMP / pharmacology
Drug Interactions
GTP-Binding Proteins / metabolism,  physiology
Leukemia, Promyelocytic, Acute / metabolism,  pathology*
Pertussis Toxin*
Phorbol Esters / pharmacology
Tretinoin / antagonists & inhibitors,  pharmacology*
Tumor Cells, Cultured / drug effects
Virulence Factors, Bordetella / pharmacology*
Reg. No./Substance:
0/Phorbol Esters; 0/Virulence Factors, Bordetella; 20762-30-5/Adenosine Diphosphate Ribose; 302-79-4/Tretinoin; 60-92-4/Cyclic AMP; EC Toxin; EC 3.6.1.-/GTP-Binding Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Presence of fructokinase in pancreatic islets.
Next Document:  Effect of the different dimeric forms of the platelet-derived growth factor on cellular responses in...