Document Detail

Inhibition by aphidicolin and dideoxythymidine triphosphate of a multienzyme complex of DNA synthesis from human cells.
MedLine Citation:
PMID:  6409668     Owner:  NLM     Status:  MEDLINE    
A multienzyme complex consisting of DNA polymerase and several DNA precursor-synthesizing enzymes was solubilized by gentle lysis of cultured human cells. This complex channelled the distal precursor [3H]dTMP into DNA. The patterns of inhibition of the complex by aphidicolin and dideoxythymidine triphosphate (ddTTP) suggested that the complex contained the replicative DNA polymerase, polymerase alpha. Inhibition by ddTTP was competitive with dTTP. This was exploited to estimate the effective concentration of [3H]dTTP at the site of DNA synthesis during channelling of [3H]dTMP into DNA. The estimated concentration (about 50 microM) was so high as to suggest that the solubilized complex was able to functionally compartmentalize DNA precursors.
R G Wickremasinghe; A V Hoffbrand
Related Documents :
23700988 - Super-resolution fluorescence imaging of directly labelled dna: from microscopy standar...
3037998 - Pyrrolo[2,3-d]pyrimidine nucleosides as inhibitors of human cytomegalovirus.
24780228 - γ-h2ax as a biomarker for dna double-strand breaks in ecotoxicology.
6700578 - Lethal effects of 1-beta-d-arabinofuranosylcytosine incorporation into deoxyribonucleic...
8628748 - The lack of efficacy of 4,6,6'-trimethylangelicin to induce immune suppression in an an...
15136738 - Inducing and modulating anisotropic dna bends by pseudocomplementary peptide nucleic ac...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  159     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1983 Aug 
Date Detail:
Created Date:  1983-09-20     Completed Date:  1983-09-20     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  175-9     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
DNA Replication / drug effects*
DNA-Directed DNA Polymerase / metabolism
Diterpenes / pharmacology*
Multienzyme Complexes / metabolism
Thymine Nucleotides / metabolism,  pharmacology*
Reg. No./Substance:
0/Dideoxynucleotides; 0/Diterpenes; 0/Multienzyme Complexes; 0/Thymine Nucleotides; 365-08-2/thymidine 5'-triphosphate; 38966-21-1/Aphidicolin; 611-60-9/2',3'-dideoxythymidine triphosphate; EC DNA Polymerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Vinculin localization in cardiac muscle.
Next Document:  Novel cell-binding activity specific for N-acetyl-D-glucosamine in an Escherichia coli strain.