| Inhibition of bombesin-stimulated gastrin release from isolated canine G cells by bombesin antagonists. | |
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MedLine Citation:
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PMID: 2483358 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study investigated the effects of two putative bombesin antagonists, [D-Arg1,D-Pro2,D-Trp7,9,Leu11]substance P and [Leu13-psi-CH2NH-Leu14]bombesin, on bombesin-stimulated gastrin release from isolated canine G cells following short-term culture. Canine antral tissue was dispersed by sequential collagenase and EDTA treatment, and counterflow elutriation was used to enrich for G cells. Plates were seeded with 2 x 10(6) cells/mL in each well and cultured for 2 days prior to testing. Gastrin-containing and somatostatin-containing cells were identified by immunocytochemistry using the biotin-avidin-peroxidase method and accounted for 8.5 and 1%, respectively, of adhered cells. Basal gastrin secretion was 1.91 +/- 0.48% of total cell content. After a 2-h incubation period, bombesin (0.01-100 pM) stimulated gastrin release in a concentration-dependent fashion. The substance P analog, at a concentration of 1 microM, modestly inhibited bombesin-stimulated gastrin release from canine G cells. This analog also produced weak stimulation of basal gastrin release. In contrast, the bombesin analog, at a concentration of 1 microM, did not affect basal gastrin secretion. The bombesin analog completely blocked bombesin-stimulated gastrin release from 0.01 to 1 pM and produced greater than 50% inhibition at higher doses. The ability of the bombesin analog to directly inhibit bombesin-stimulated gastrin release from cultured canine G cells underscores its usefulness in studies involving the role of bombesin and its mammalian counterpart, gastrin-releasing peptide, in the control of gastrin cell function. |
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Authors:
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R V Campos; A M Buchan; R A Pederson; C H McIntosh; D H Coy |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Canadian journal of physiology and pharmacology Volume: 67 ISSN: 0008-4212 ISO Abbreviation: Can. J. Physiol. Pharmacol. Publication Date: 1989 Dec |
Date Detail:
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Created Date: 1990-04-23 Completed Date: 1990-04-23 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0372712 Medline TA: Can J Physiol Pharmacol Country: CANADA |
Other Details:
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Languages: eng Pagination: 1520-4 Citation Subset: IM |
Affiliation:
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Department of Physiology, University of British Columbia, Vancouver, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bombesin / analogs & derivatives, antagonists & inhibitors, pharmacology* Dogs Gastric Mucosa / cytology, drug effects, metabolism* Gastrin-Releasing Peptide Gastrins / metabolism* Immunohistochemistry Peptides / metabolism* Radioimmunoassay Recombinant Proteins* Somatostatin / immunology, secretion Substance P / analogs & derivatives, pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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CA-45153/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Gastrins; 0/Peptides; 0/Recombinant Proteins; 115136-07-7/bombesin, Leu(13)-psi-CH2NH-Leu(14)-; 31362-50-2/Bombesin; 33507-63-0/Substance P; 51110-01-1/Somatostatin; 80043-53-4/Gastrin-Releasing Peptide; 83374-71-4/substance P, Arg(1)-Pro(2)-Trp(7,9)-Leu(11)- |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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