Document Detail


Inhibition of boar sperm hyaluronidase activity by tannic acid reduces polyspermy during in vitro fertilization of porcine oocytes.
MedLine Citation:
PMID:  17266786     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study was conducted to examine the effects of three polyphenols (tannic acid, apigenin and quercetin) on hyaluronidase activity and in vitro fertilization (IVF) parameters. Among them, tannic acid showed by far the strongest potency for blocking hyaluronidase activity extracted from preincubated boar sperm, causing a dose-dependent inhibition over the range of 2-10 microg/ml. When cumulus-intact and cumulus-free oocytes were inseminated in IVF medium containing tannic acid, the penetration and the polyspermy rates were significantly decreased in the presence of 10 microg/ml tannic acid compared with those in the absence of tannic acid, and the addition of 5 microg/ml tannic acid significantly reduced the polyspermy rate (p < 0.05) compared with that of the control while maintaining the high penetration rate. However, apigenin and quercetin had no effect on the rate of polyspermy. Interestingly, the incidence of polyspermy was significantly reduced in oocytes inseminated with sperm pretreated with 5 microg/ml tannic acid (p < 0.05), although the pretreatment of oocytes had no effect against the polyspermy after insemination with untreated sperm. Treatment with tannic acid caused neither a protective proteolytic modification of the zona pellucida matrix before fertilization, nor a reduction of the proteolytic activity of acrosomal contents or the number of zona-bound spermatozoa. These data suggest that an appropriate concentration of tannic acid prevents polyspermy through the inhibition of sperm hyaluronidase activity during IVF of porcine oocytes.
Authors:
Hideki Tatemoto; Isao Tokeshi; Satoshi Nakamura; Norio Muto; Tadashi Nakada
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Zygote (Cambridge, England)     Volume:  14     ISSN:  0967-1994     ISO Abbreviation:  Zygote     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2007-02-01     Completed Date:  2007-03-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9309124     Medline TA:  Zygote     Country:  England    
Other Details:
Languages:  eng     Pagination:  275-85     Citation Subset:  IM    
Affiliation:
Department of Bioproduction, University of Ryukyus, Okinawa, Japan. hidettmt@agr.u-ryukyu.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Apigenin / pharmacology
Dose-Response Relationship, Drug
Enzyme Inhibitors / pharmacology
Female
Fertilization / drug effects*,  physiology
Fertilization in Vitro / methods
Hyaluronoglucosaminidase / antagonists & inhibitors*
Male
Oocytes / drug effects
Quercetin / pharmacology
Sperm-Ovum Interactions / drug effects
Spermatozoa / drug effects*,  enzymology*
Sus scrofa
Tannins / administration & dosage,  pharmacology*
Zona Pellucida / drug effects
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Tannins; 117-39-5/Quercetin; 520-36-5/Apigenin; EC 3.2.1.35/Hyaluronoglucosaminidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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