Document Detail


Inhibition of ataxia-telangiectasia mutated by antisense oligonucleotide nanoparticles induces radiosensitization of head and neck squamous-cell carcinoma in mice.
MedLine Citation:
PMID:  19435407     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ataxia-telangiectasia-mutated (ATM) is a radiosensitization gene. In the present study, we investigated the efficacy of poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles containing ATM antisense oligonucleotides (ASOs) for the radiosensitization of head and neck squamous-cell carcinoma in mice, using the SCCVII cell line. Nanoparticles containing ATM ASOs were prepared with PLGA by using a double-emulsion solvent evaporation method. The results showed that the nanoparticles were suitable for intracellular uptake, and ATM ASOs inhibited ATM expression when delivered by using nanoparticles or lipofectin, but not in their free form. Meanwhile, we found that ATM reduction sensitized SCCVII cells in vitro and tumors in vivo to irradiation. In conclusion, biodegradable PLGA nanoparticles, used as a delivery carrier, enhanced intracellular uptake of ATM ASOs into SCCVII cells and the inhibitory effect of ATM ASOs. These results demonstrated that antisense ATM therapy, using PLGA nanoparticles, might provide a therapeutic benefit to patients undergoing radiation therapy for head and neck squamous-cell carcinoma.
Authors:
Jian Zou; Xiaoming Qiao; Huiping Ye; Yi Zhang; Junming Xian; Houyu Zhao; Shixi Liu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer biotherapy & radiopharmaceuticals     Volume:  24     ISSN:  1557-8852     ISO Abbreviation:  Cancer Biother. Radiopharm.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-06-22     Completed Date:  2009-09-28     Revised Date:  2011-11-02    
Medline Journal Info:
Nlm Unique ID:  9605408     Medline TA:  Cancer Biother Radiopharm     Country:  United States    
Other Details:
Languages:  eng     Pagination:  339-46     Citation Subset:  IM    
Affiliation:
Department of Otolaryngology, West China Hospital, Sichuan University, Chengdu, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carcinoma, Squamous Cell / pathology,  radiotherapy*
Cell Cycle Proteins / antagonists & inhibitors*,  genetics,  metabolism
Cell Line, Tumor
Cell Survival / radiation effects
DNA-Binding Proteins / antagonists & inhibitors*,  genetics,  metabolism
Endocytosis / physiology
Female
Gene Expression / drug effects
Head and Neck Neoplasms / pathology,  radiotherapy*
Humans
Mice
Mice, Inbred C3H
Mutagenesis / drug effects*
Nanoparticles / chemistry*
Oligonucleotides, Antisense / administration & dosage,  pharmacology*
Particle Size
Protein-Serine-Threonine Kinases / antagonists & inhibitors*,  genetics,  metabolism
Radiation Tolerance / drug effects*
Radiation-Sensitizing Agents / administration & dosage,  pharmacology
Tumor Suppressor Proteins / antagonists & inhibitors*,  genetics,  metabolism
X-Rays
Xenograft Model Antitumor Assays
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/DNA-Binding Proteins; 0/Oligonucleotides, Antisense; 0/Radiation-Sensitizing Agents; 0/Tumor Suppressor Proteins; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/ataxia telangiectasia mutated protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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