Document Detail


Inhibition of aromatase activity by 8-bromo-cyclic adenosine monophosphate in cultured first-trimester human trophoblast.
MedLine Citation:
PMID:  2240104     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have previously shown that 8-bromo-adenosine-3':5'-cyclic monophosphate inhibits production of estradiol from androgen precursors in trophoblast isolated from term placentas. In these experiments we sought to evaluate the effect of 8-bromo-adenosine-3':5'-cyclic monophosphate on aromatase activity in first-trimester trophoblast culture. Trophoblast cells were enzyme dispersed from first-trimester placentas and purified on a Percoll gradient. On the third and fourth day after dispersion cultures were treated with androstenedione or testosterone with and without 2 mmol/L 8-bromo-adenosine-3':5'-cyclic monophosphate. The quantity of estradiol, estrone, progesterone, human placental lactogen, and human chorionic gonadotropin was determined by radioimmunoassay. We concluded that in first-trimester trophoblast, as previously reported in term trophoblast, 8-bromo-adenosine-3':5'-cyclic monophosphate inhibited the production of estradiol from the supplied androgen precursors. Human chorionic gonadotropin and human placental lactogen production was stimulated by 8-bromo-adenosine-3':5'-cyclic monophosphate. Progesterone production was either stimulated or was unchanged by the presence of 8-bromo-adenosine-3':5'-cyclic monophosphate.
Authors:
M R Rodway; B H Yuen; P C Leung
Related Documents :
24921924 - The role of platelets in the pathogenesis of viral hemorrhagic fevers.
23228564 - Fkbp52 is involved in the regulation of soce channels in the human platelets and meg 01...
24390064 - Platelet activation and platelet-leukocyte aggregation elicited in experimental colitis...
16413094 - Carbaryl inhibits basal and fsh-induced progesterone biosynthesis of primary human gran...
8137884 - Nucleoside transport inhibition and platelet aggregation in human blood: r75231 and its...
22557824 - One-step apexification in immature tooth using grey mineral trioxide aggregate as an ap...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of obstetrics and gynecology     Volume:  163     ISSN:  0002-9378     ISO Abbreviation:  Am. J. Obstet. Gynecol.     Publication Date:  1990 Nov 
Date Detail:
Created Date:  1990-12-14     Completed Date:  1990-12-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370476     Medline TA:  Am J Obstet Gynecol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1546-51     Citation Subset:  AIM; IM    
Affiliation:
Department of Obstestrics and Gynecology, University of British Columbia, Grace Hospital, Vancouver, Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
8-Bromo Cyclic Adenosine Monophosphate / pharmacology*
Androstenedione / metabolism
Aromatase Inhibitors*
Cells, Cultured
Chorionic Gonadotropin / biosynthesis
Estradiol / biosynthesis
Estrone / biosynthesis
Female
Humans
Placental Lactogen / biosynthesis
Pregnancy
Pregnancy Trimester, First
Progesterone / biosynthesis
Testosterone / metabolism
Trophoblasts / drug effects*,  enzymology,  metabolism
Chemical
Reg. No./Substance:
0/Aromatase Inhibitors; 0/Chorionic Gonadotropin; 23583-48-4/8-Bromo Cyclic Adenosine Monophosphate; 50-28-2/Estradiol; 53-16-7/Estrone; 57-83-0/Progesterone; 58-22-0/Testosterone; 63-05-8/Androstenedione; 9035-54-5/Placental Lactogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The perinatal impact of cocaine, amphetamine, and opiate use detected by universal intrapartum scree...
Next Document:  A reappraisal of the need for autologous blood donation in the obstetric patient.