Document Detail

Inhibition of aromatase activity by 8-bromo-cyclic adenosine monophosphate in cultured first-trimester human trophoblast.
MedLine Citation:
PMID:  2240104     Owner:  NLM     Status:  MEDLINE    
We have previously shown that 8-bromo-adenosine-3':5'-cyclic monophosphate inhibits production of estradiol from androgen precursors in trophoblast isolated from term placentas. In these experiments we sought to evaluate the effect of 8-bromo-adenosine-3':5'-cyclic monophosphate on aromatase activity in first-trimester trophoblast culture. Trophoblast cells were enzyme dispersed from first-trimester placentas and purified on a Percoll gradient. On the third and fourth day after dispersion cultures were treated with androstenedione or testosterone with and without 2 mmol/L 8-bromo-adenosine-3':5'-cyclic monophosphate. The quantity of estradiol, estrone, progesterone, human placental lactogen, and human chorionic gonadotropin was determined by radioimmunoassay. We concluded that in first-trimester trophoblast, as previously reported in term trophoblast, 8-bromo-adenosine-3':5'-cyclic monophosphate inhibited the production of estradiol from the supplied androgen precursors. Human chorionic gonadotropin and human placental lactogen production was stimulated by 8-bromo-adenosine-3':5'-cyclic monophosphate. Progesterone production was either stimulated or was unchanged by the presence of 8-bromo-adenosine-3':5'-cyclic monophosphate.
M R Rodway; B H Yuen; P C Leung
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of obstetrics and gynecology     Volume:  163     ISSN:  0002-9378     ISO Abbreviation:  Am. J. Obstet. Gynecol.     Publication Date:  1990 Nov 
Date Detail:
Created Date:  1990-12-14     Completed Date:  1990-12-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370476     Medline TA:  Am J Obstet Gynecol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1546-51     Citation Subset:  AIM; IM    
Department of Obstestrics and Gynecology, University of British Columbia, Grace Hospital, Vancouver, Canada.
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MeSH Terms
8-Bromo Cyclic Adenosine Monophosphate / pharmacology*
Androstenedione / metabolism
Aromatase Inhibitors*
Cells, Cultured
Chorionic Gonadotropin / biosynthesis
Estradiol / biosynthesis
Estrone / biosynthesis
Placental Lactogen / biosynthesis
Pregnancy Trimester, First
Progesterone / biosynthesis
Testosterone / metabolism
Trophoblasts / drug effects*,  enzymology,  metabolism
Reg. No./Substance:
0/Aromatase Inhibitors; 0/Chorionic Gonadotropin; 23583-48-4/8-Bromo Cyclic Adenosine Monophosphate; 50-28-2/Estradiol; 53-16-7/Estrone; 57-83-0/Progesterone; 58-22-0/Testosterone; 63-05-8/Androstenedione; 9035-54-5/Placental Lactogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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