Document Detail


Inhibition of apoptosis in cultured immature rat Leydig cells by human chorionic gonadotropin associated with Bcl-2 mRNA expression.
MedLine Citation:
PMID:  10711723     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bcl-2 is the first identified negative regulator of apoptotic cell death. When the level of Bcl-2 mRNA in rat whole testes was examined in the present study, it gradually decreased in rats from 2.5 to 9 weeks old. We also examined the effect of human chorionic gonadotropin (hCG) on apoptosis and the level of Bcl-2 mRNA expression in immature Leydig cells in vitro. When the cells were cultured with serum free media (SFM), Bcl-2 mRNA levels gradually decreased. On the other hand, the level of Bcl-2 mRNA in cells treated with 50 ng/ml of hCG decreased at 6 h, but increased after 12 h. At 24 h, the level of Bcl-2 mRNA in the treated cells was almost the same as that of control cells (time = 0). At 12 h after the addition of various concentrations (from 0.1-1000 ng/ml) of hCG, Bcl-2 mRNA levels increased in a dose-dependent manner. An analysis of DNA fragmentation showed that treatment with hCG prevents the apoptosis of immature Leydig cells. Our findings suggest that Bcl-2 mRNA may be related to the programmed cell death of immature rat Leydig cells in vitro, which are inhibited by hCG.
Authors:
M Fujisawa; M Kanzaki; N Tatsumi; Y Okuda; H Okada; S Arakawa; S Kamidono
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Endocrine research     Volume:  26     ISSN:  0743-5800     ISO Abbreviation:  Endocr. Res.     Publication Date:  2000 Feb 
Date Detail:
Created Date:  2000-03-30     Completed Date:  2000-03-30     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8408548     Medline TA:  Endocr Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  59-70     Citation Subset:  IM    
Affiliation:
Department of Urology, Kobe University School of Medicine, Japan. masato@med.kobe-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn / physiology
Apoptosis / drug effects*,  physiology
Cells, Cultured
Chorionic Gonadotropin / pharmacology*
Culture Media, Serum-Free / pharmacology
DNA Fragmentation / drug effects
Dose-Response Relationship, Drug
Humans
Leydig Cells / drug effects,  metabolism,  physiology*
Male
Proto-Oncogene Proteins c-bcl-2 / genetics*
RNA, Messenger / metabolism*
Rats
Rats, Sprague-Dawley
Testis / metabolism
Time Factors
Chemical
Reg. No./Substance:
0/Chorionic Gonadotropin; 0/Culture Media, Serum-Free; 0/Proto-Oncogene Proteins c-bcl-2; 0/RNA, Messenger

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