| Inhibition of apoptosis by hyperbaric oxygen in a rat focal cerebral ischemic model. | |
| | |
MedLine Citation:
|
PMID: 12843789 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The hypothesis was tested that hyperbaric oxygen therapy (HBO) reduced brain infarction by preventing apoptotic death in ischemic cortex in a rat model of focal cerebral ischemia. Male Sprague-Dawley rats were subjected to middle cerebral artery occlusion/reperfusion (MCAO/R) and subsequently were exposed to HBO (2.5 atmospheres absolute) for 2 h, at 6 h after reperfusion. Rats were killed and brain samples were collected at 24, 48, 72 h, and 7 days after reperfusion. Neurologic deficits, infarction area, and apoptotic changes were evaluated by clinical scores, 2,3,7-triphenyltetrazolium chloride staining, caspase-3 expression, DNA fragmentation assay, and terminal deoxynucleotidyl transferase-mediated 2'-deoxyuridine 5'-triphosphate-biotin nick end labeling (TUNEL)-hematoxylin and eosin (H&E) costaining. In MCAO/R without HBO treatment animals, DNA fragmentation was observed in injured cortex at 24, 48, and 72 h but not in samples at 7 days after reperfusion. Double labeling of brain slides with NeuN and caspase-3 demonstrated neurons in the injured cortex labeled with caspase-3. TUNEL+H&E costaining revealed morphologic apoptotic changes at 24, 48, and 72 h after reperfusion. Hyperbaric oxygen therapy abolished DNA fragmentation and reduced the number of TUNEL-positive cells. Hyperbaric oxygen therapy reduced infarct area and improved neurologic scores at 7 days after reperfusion. One of the molecular mechanisms of HBO-induced brain protection is to prevent apoptosis, and this effect of HBO might preserve more brain tissues and promote neurologic functional recovery. |
| | |
Authors:
|
Dali Yin; Changman Zhou; Ikuyo Kusaka; John W Calvert; Andrew D Parent; Anil Nanda; John H Zhang |
Related Documents
:
|
2674169 - Effect of the nmda antagonist mk-801 on local cerebral blood flow in focal cerebral isc... 16220079 - Chronic at1 receptor blockade alters the mechanisms mediating hypoxic dilation in middl... 23635089 - Study of the protective effect of vitamin c on testicular tissue following experimental... 8552429 - L-name reduces infarct volume in a filament model of transient middle cerebral artery o... 17946879 - A pilot studies in dynamic profile of multi parameters of eeg in a rat model of transie... 23178799 - Psychoactive "bath salts": not so soothing. 10095039 - Fluoxetine-induced plasticity in the rodent visual system. 2674169 - Effect of the nmda antagonist mk-801 on local cerebral blood flow in focal cerebral isc... 20474069 - Resveratrol ameliorates vasculopathy in stz-induced diabetic rats: role of age-rage sig... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism Volume: 23 ISSN: 0271-678X ISO Abbreviation: J. Cereb. Blood Flow Metab. Publication Date: 2003 Jul |
Date Detail:
|
Created Date: 2003-07-04 Completed Date: 2003-08-26 Revised Date: 2005-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 8112566 Medline TA: J Cereb Blood Flow Metab Country: United States |
Other Details:
|
Languages: eng Pagination: 855-64 Citation Subset: IM |
Affiliation:
|
Department of Neurosurgery, University of Mississippi Medical Center, Jackson, Mississippi, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Apoptosis* Brain / metabolism, pathology* Brain Ischemia / pathology, physiopathology, therapy* Caspases / metabolism Coloring Agents / metabolism DNA Fragmentation Disease Models, Animal Hyperbaric Oxygenation* In Situ Nick-End Labeling Infarction, Middle Cerebral Artery Male Neuropsychological Tests Oxygen / metabolism* Random Allocation Rats Rats, Sprague-Dawley Tetrazolium Salts / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Coloring Agents; 0/Tetrazolium Salts; 7782-44-7/Oxygen; 902-00-1/triphenyltetrazolium; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Accelerated glial reactivity to stroke in aged rats correlates with reduced functional recovery.
Next Document: Persistently low extracellular glucose correlates with poor outcome 6 months after human traumatic b...