Document Detail

Inhibition of apoptosis by expression of antiapoptotic proteins in recombinant human keratinocytes.
MedLine Citation:
PMID:  17912957     Owner:  NLM     Status:  MEDLINE    
The Fas ligand/Fas interaction plays an important role in the regulation of immune responses. Allografted cells undergo Fas-mediated apoptosis induced by CD8+ T cells. Our objective was to prevent human keratinocytes from immunologically induced apoptosis. We focused on three proteins with inhibitory function on Fas-mediated apoptosis. Human keratinocytes were transfected with either Flip, Faim, or Lifeguard (LFG). The treatment proved to be practicable and efficient. The recombinant keratinocytes with expression of our target proteins were cocultured with CD8+ T cells and the apoptotic activity was then evaluated. Activation of caspase-8 was detectable in control but not in the recombinant cells. Quantitative analysis revealed significant induction of T-cell-induced apoptosis in nontransfected keratinocytes (p = 0.04, n = 12) but not in Flip (p = 0.66), Faim (p = 0.42), or LFG (p = 0.44) expressing cells. Our results suggest that heterotopic expression of antiapoptotic proteins can induce the resistance of keratinocytes to a major mechanism of rejection.
Claudia Y U Choi; Kerstin Reimers; Christina Allmeling; Susanne Kall; Yeong-Hoon Choi; Peter M Vogt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell transplantation     Volume:  16     ISSN:  0963-6897     ISO Abbreviation:  Cell Transplant     Publication Date:  2007  
Date Detail:
Created Date:  2007-10-04     Completed Date:  2007-11-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9208854     Medline TA:  Cell Transplant     Country:  United States    
Other Details:
Languages:  eng     Pagination:  663-74     Citation Subset:  IM    
Department of Plastic, Hand and Reconstructive Surgery, Medical School Hannover, D-30625 Hannover, Germany.
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MeSH Terms
Antigens, CD8 / immunology*
Antigens, CD95 / immunology
Apoptosis / drug effects,  immunology*
Apoptosis Regulatory Proteins / immunology*,  metabolism,  pharmacology
CASP8 and FADD-Like Apoptosis Regulating Protein / metabolism,  pharmacology
CD8-Positive T-Lymphocytes / immunology*
Cell Survival / immunology
Cells, Cultured
Coculture Techniques
Fas Ligand Protein / immunology
Feasibility Studies
Gene Expression
Graft Rejection / prevention & control*
Keratinocytes / drug effects,  immunology*,  metabolism
Membrane Proteins / metabolism,  pharmacology
Recombinant Proteins / pharmacology
Reg. No./Substance:
0/Antigens, CD8; 0/Antigens, CD95; 0/Apoptosis Regulatory Proteins; 0/CASP8 and FADD-Like Apoptosis Regulating Protein; 0/FAIM protein, human; 0/FAIM2 protein, human; 0/Fas Ligand Protein; 0/Membrane Proteins; 0/Recombinant Proteins

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