Document Detail

Inhibition of antibody binding to viable cells by liposomes containing purified rat spleen glycoprotein.
MedLine Citation:
PMID:  6655237     Owner:  NLM     Status:  MEDLINE    
The capacity of liposomes with inserted RT-1 histocompatibility antigen to bind anti-RT-1 antibodies varies depending on their lipid composition and mode of preparation. The binding capacity of liposomes prepared by dialysis is different from that of liposomes prepared by gel filtration or of small membrane protein micelles. A new assay for analysis of membrane antigens has been developed. Liposomes are used to compete for antibody binding with in vitro cultured cells while binding of antibody to the same adherent liposomes is simultaneously assayed.
B Jansson; H O Sjögren
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of immunological methods     Volume:  65     ISSN:  0022-1759     ISO Abbreviation:  J. Immunol. Methods     Publication Date:  1983 Dec 
Date Detail:
Created Date:  1984-02-14     Completed Date:  1984-02-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  1305440     Medline TA:  J Immunol Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  159-69     Citation Subset:  IM    
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MeSH Terms
Antigens, Surface / immunology
Binding Sites, Antibody*
Binding, Competitive
Cell Line
Glycoproteins / immunology*
Histocompatibility Antigens / analysis,  immunology
Immunoassay / methods*
Liposomes / analysis,  immunology*
Membrane Proteins / immunology*
Osmolar Concentration
Rats, Inbred BN
Rats, Inbred WF
Spleen / analysis
Grant Support
Reg. No./Substance:
0/Antigens, Surface; 0/Binding Sites, Antibody; 0/Glycoproteins; 0/Histocompatibility Antigens; 0/Liposomes; 0/Membrane Proteins

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