|Inhibition of androstenediol-dependent LNCaP tumour growth by 17alpha-ethynyl-5alpha-androstane-3alpha, 17beta-diol (HE3235).|
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|PMID: 19337256 Owner: NLM Status: MEDLINE|
|Androst-5-ene-3beta, 17beta-diol (AED) is an adrenal hormone that has been reported to sustain prostate cancer growth after androgen deprivation therapy (ADT). LNCaP cells express a mutated androgen receptor that confers the ability to respond not only to androgen but also to oestrogen and adrenal hormones such as AED, and thus provide a cell line useful for identifying compounds capable of inhibiting AED-stimulated cell growth. We sought to determine whether structurally related steroids could inhibit AED-stimulated LNCaP cell growth in vitro and tumour growth in vivo. We report here the identification of a novel androstane steroid, HE3235 (17alpha-ethynyl-5alpha-androstan-3alpha, 17beta-diol), with significant inhibitory activity for AED-stimulated LNCaP proliferation. This inhibitory activity is accompanied by an increase in the number of apoptotic cells. Animal studies have confirmed the cytoreductive activity of HE3235 on LNCaP tumours. The results suggest that this compound may be of clinical use in castration-resistant prostate cancer.|
|R Trauger; E Corey; D Bell; S White; A Garsd; D Stickney; C Reading; J Frincke|
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|Type: Journal Article|
|Title: British journal of cancer Volume: 100 ISSN: 1532-1827 ISO Abbreviation: Br. J. Cancer Publication Date: 2009 Apr|
|Created Date: 2009-04-01 Completed Date: 2009-04-24 Revised Date: 2013-06-02|
Medline Journal Info:
|Nlm Unique ID: 0370635 Medline TA: Br J Cancer Country: England|
|Languages: eng Pagination: 1068-72 Citation Subset: IM|
|Hollis-Eden Pharmaceuticals, San Diego, CA 92121, USA. email@example.com|
|APA/MLA Format Download EndNote Download BibTex|
Androstenediol / pharmacology*
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects
Cell Line, Tumor
Cell Proliferation / drug effects
Prostatic Neoplasms / drug therapy*, pathology
Receptors, Androgen / genetics
|0/17-ethynylandrostane-3,17-diol; 0/Androstanols; 0/Antineoplastic Agents; 0/Receptors, Androgen; 521-17-5/Androstenediol|
Journal ID (nlm-ta): Br J Cancer
Publisher: Nature Publishing Group
Copyright 2009, Cancer Research UK
Received Day: 09 Month: 12 Year: 2008
Revision Received Day: 18 Month: 02 Year: 2009
Accepted Day: 18 Month: 02 Year: 2009
Electronic publication date: Day: 31 Month: 03 Year: 2009
Print publication date: Day: 07 Month: 04 Year: 2009
Volume: 100 Issue: 7
First Page: 1068 Last Page: 1072
Publisher Item Identifier: 6604987
PubMed Id: 19337256
|Inhibition of androstenediol-dependent LNCaP tumour growth by 17α-ethynyl-5α-androstane-3α, 17β-diol (HE3235)|
1Hollis-Eden Pharmaceuticals San Diego, CA, USA
2Department of Urology, University of Washington Seattle WA, USA
3Merck & Co Inc. North Wales, PA, USA
|*Author for correspondence: firstname.lastname@example.org
A considerable amount of research has been directed towards understanding factors that support the growth and expansion of prostate cancer during and after anti-androgen treatment. The observation that androgen deprivation results in increase in AR expression (Scher et al, 2004) highlights the importance of AR in this stage of disease and suggests that other ligands may be capable of binding and activating AR to drive tumour cell growth. It is well documented that mutations in the AR allow castration-resistant prostate cancer cells to utilise a broad spectrum of steroids, whose levels are not affected by anti-androgen therapy (Taplin et al, 1995). These include oestrogen, progesterone, glucocorticoid, and the adrenal hormone androst-5-ene-3β, 17β-diol (AED), a multi-potent hormone that is an androgen and oestrogen precursor, with the ability to directly transactivate AR and ER in prostate cancer cells (Miyamoto et al, 1998; Maggiolini et al, 2004).
Because AED's central role both as a precursor of DHT and a direct activator of the androgen receptor, we initiated studies to identify an inhibitor of AED signalling in hormonally sensitive tumour cells from a proprietary chemical library based on the structure of AED. Through the course of this work, compounds were identified that inhibited the proliferation of LNCaP cells stimulated by AED in vitro. Subsequent work to identify the most suitable pharmaceutical from this series of compounds resulted in the identification of HE3235 (17α-ethynyl-5α-androstan-3α, 17β-diol). Here, we present results showing that HE3235 blocks the growth of LNCaP cells by inducing apoptosis. Tumour studies have confirmed the in vivo activity of HE3235 against AED-stimulated LNCaP tumour cells.
HE3235 was synthesised by reacting trimethylsilylazine with androsterone (DGP, Shanghai, China) in refluxing acetonitrile using saccharin catalyst. The trimethylsilyl-protected intermediate was subsequently reacted with ethynyltrimethylsilane activated with n-butyl lithium in tetrahydrofuran. The crude product was then recovered by slow addition of water to quench the reaction and filtration of the crude precipitate. The reaction product was purified to >99% pure HE3235 with a 67% of overall yield.
LNCaP-FGC, PC3, and DU145 cells were obtained from the American Type Tissue Collection (ATTC, Manassas, VA, USA). To identify compounds with anti-proliferative activity for LNCaP, cells were seeded in poly-D-lysine coated 96-well plates at 3.75 × 104 per ml in a total volume of 200 μl of RPMI per 10% foetal bovine serum (FBS). After incubation overnight at 37°C, 5% CO2, the media was removed and the cells were washed once with 150 μl per well of phenol red-free RPMI containing 10% charcoal per dextran-treated FBS (CS-RPMI) containing 10 nM AED. The appropriate dilution of each test compound was added and the cells were incubated for 5 days at 37°C, 5% CO2. For IC50 determination, a dilution sequence of compound was analysed and the data were modelled using a nonlinear regression, sigmoidal dose response, variable slope equation. The curve fit was constrained with a bottom of 0 and a top of 100. IC50 values were generated by GraphPad Prism 4. Cell proliferation was measured on day 5 using the CyQuant assay (Invitrogen, Carlsbad, CA, USA). The affect of HE3235 on cell growth of LNCaP, PC3, and Du145 cells was assayed by plating 3 × 105 cells in 5% CS-RPMI supplemented with 10 nM AED. After 4 days, cells were manually counted with a haemocytometer.
LNCaP cells (5 × 105) were seeded in phenol red-free RPMI with 5% charcoal-stripped serum (CSS) in 6-well plates and allowed to adhere overnight, then cultured in CSS supplemented with 10 nM AED with or without 50 nM HE3235 for 4 days. At the end of the incubation period, floating and adherent cells were harvested for analysis. Apoptosis was measured using the Annexin V-FITC Apoptosis Detection Kit (Calbiochem, La Jolla, CA, USA) according to the instructions of the manufacturer to detect apoptosis. PI and Annexin positive cells were considered apoptotic.
The ARE-luciferase reporter construct (probasin promoter containing ARE) was provided by Dr Plymate (University of Washington, Seattle, WA, USA), and the 5.8 kb PSA promoter-luciferase plasmid was provided by Dr Kim (Fred Hutchinson Cancer Research Center, Seattle, WA, USA). An Amaxa Nucleofector device was used for transfection of LNCaP cells using solution V and program T-27 according to the manufacturer's directions (Amaxa Biosystems Inc., Gaithersburg, MD, USA). LNCaP cells (1 × 106) were transfected with ARE-reporter plasmid or PSA-promoter reporter plasmid (1 μg). The hTK renilla-luciferase plasmid (2 ng) was transfected under the same conditions to enable normalisation of transfection efficiencies. Cells were seeded in 24-well plates in phenol red-free RPMI with 5% CSS to allow adherence overnight, and then DHT, E2, or AED were added (1 nM or 10 nM final concentration) with or without 50 nM HE3235 in DMSO. Cells were incubated for ∼48 h, lysed and subjected to a dual-luciferase assay according to manufacturer's recommendations (Promega, Madison, WI, USA) using Tecan Genios plus luminometer. The luciferace activity was measured and signal normalised to transfection efficiencies based on TK transfection.
All animal studies were performed within the established IACUC guidelines. Castrated SCID mice (6 week old) were obtained from Jackson Laboratories (Bar Harbor, ME, USA). After 4 days of acclimation, the mice were implanted with AED pellets (5 mg, 60-day time-release, IRA, Sarasota, FL, USA). After 3 days, all mice were injected subcutaneously in the right flank with 100 μl of 7.5 × 106 LNCaP tumour cells in phenol red-free RPMI mixed 1 : 1 with Matrigel (BD, Franklin Lakes, NJ, USA). Tumour volumes were measured weekly and calculated as a2 × b/2 with a being the width and b the length of the tumour in mm (reported as mm3). HE3235 was prepared for injection by dilution in Captisol (CyDex, Lenexa, KS, USA).
To test the effect of dose on tumour incidence, a total of 48 castrated male SCID mice were implanted with LNCaP tumour cells as described above, and the mice were randomized into four groups of 12 animals each (vehicle control, 4 mg per mouse per day, 1 mg per mouse per day, and 0.4 mg per mouse per day). Administration of HE3235 (200 μl intraperitoneally) or vehicle began 24 h after tumour inoculation. All animals were dosed daily for 28 consecutive days.
To test the effect of HE3235 on established LNCaP tumours, 36 castrated SCID mice received AED pellets and were implanted with LNCaP tumour cells and monitored as described above. Once the tumours reached 15–25 mm3, the mice were paired by tumour volume, and each mouse in a pair was assigned to either the vehicle or 4 mg per mouse per day HE3235 group. Animals were dosed IP with 200 μl HE3235 or vehicle once a day for 21 days.
The incidence of a measurable tumour was estimated as the relative frequency of mice having a measurable tumour at least once, any time in the experiment. The significance of the difference in incidence from vehicle was tested by means of Fisher's exact test, adjusted for multiplicity of comparisons (Westfall et al, 1999). Dose response in the proportion of mice with tumour was tested for significance through exact Cochran–Armitage test (StatXact 2004). Time to first measurable tumour volume is analysed through Kaplan–Meier product-limit estimates, with the exact logrank test applied to test for the significance of the differences (Cantor, 1997). Tumour volumes and tumour growth rates are analysed non-parametrically through exact Wilcoxon–Mann–Whitney test. Resulting P-values are corrected for multiplicity of comparisons (Westfall et al, 1999).
Reduction of tumour volume refers to a reduction persisting to the end of the study. Fisher's exact test was applied to test the significance of the difference in the incidence of such reduction relative to vehicle.
Time to first measurable tumour volume is analysed through Kaplan–Meier product-limit estimates, with the exact logrank test applied to test for the significance of the difference. Reduction of tumour volume is defined as a reduction in volume of at least 20% of the baseline volume, persisting to the end of the study. To detect the difference between active and control group, Fisher's exact test and exact 95% CI for the difference are applied. A tumour of non-measurable volume is a tumour that, with the methodology at hand, measures 0 to the end of the study. The growth rate of a tumour is also analysed through the mixed model.
Through titration experiments, a concentration of 10 nM AED was found to provide maximal stimulation of LNCaP, and no proliferation of LNCaP cells was observed without the addition of AED (data not shown). Compounds ranked for potency by titration to calculate the IC50 of the compound. One compound, HE3235, inhibited LNCaP proliferation in the low nanomolar range (6 nM). This compound reduced LNCaP levels by 60% but had no inhibitory effect on the AR-negative PC3 and DU145 cells (Figure 1).
Treatment with HE3235 resulted in a significant reduction in tumour incidence (compared with vehicle) in the two highest dose groups, (40 mg kg−1, P=0.006, n=11; 160 mg kg−1, P<0.001, n=12), with decreases in tumour volume apparent in all three dose groups (Figure 2). The mean tumour volume in the animals that developed tumours were also significantly affected, 157 mm3 (±153) (vehicle) versus 0 (P<0.001), 4 (±7) (P<0.001), 34 (±41) (P=0.004) mm3 in the HE3235 treated animals (descending dose). There was also a statistically significant delay in the time to a measurable tumour volume in all treated groups (P<0.01) relative to the vehicle control group.
Anti-tumor activity was also observed in animals with established tumours. As seen in Figure 3, vehicle-treated animals showed a progressive increase in tumour volume over the course of the study. In contrast, HE3235 treatment significantly blocked the growth of tumours (P<0.001). Significant differences in tumour volumes between the control and treated groups were observed by the first week and were maintained through the course of this study (P<0.001). A significantly greater percentage of mice in the treatment group reduced their tumour volumes by 20% or more (P<0.03), whereas no tumour reduction was seen in the vehicle group. Furthermore, tumours in two animals receiving HE3235 became non-measurable by day 15 of the study.
To determine whether HE3235 inhibited the activation of the LNCaP AR, LNCaP cells were transfected with an ARE and incubated with HE3235 with or without DHT and AED. As can be seen in Figure 4, HE3235 did not inhibit the activation of the LNCaP AR when the cells were stimulated with either DHT or AED. Instead, HE3235 activated AR in this setting. Consistent with this finding, an increase in amount of PSA secretion per cell was observed after incubation with HE3235. PSA rose from 8.5 ng per ml per 105 cells to 175 ng per ml per 105 cells by day 4 in LNCaP cells cultures incubated with 50 nM HE3235.
Based on the observations above, the possibility that HE3235 was affecting cell viability in LNCaP cells was examined. Figure 5 shows a modest increase (8–21%) in the percentage of apoptotic LNCaP cells after 4 days of culture with HE3235, suggesting that HE3235 is cytotoxic for LNCaP cells.
The human prostate cancer cell-line LNCaP expresses a mutant androgen receptor that allows it to respond to a wide variety of hormones, including the adrenal hormone AED (Miyamoto et al, 1998; Chang et al, 1999; Mizokami et al, 2004; He and Falany, 2007). Because of its potential role in sustaining prostate cancer cell growth after androgen deprivation therapy (ADT), we set out to determine whether a compound capable of inhibiting AED-stimulated LNCaP cell proliferation could be identified from a proprietary steroid library that was developed around the structure of AED. As described here, this library yielded a novel androstane structure, HE3235, which blocked the proliferation of AED-stimulated LNCaP cells. This compound had no inhibitory effect on the AR-negative PC3 and DU145 cells (data not shown), suggesting that expression of the AR was required for inhibitory activity. When administered to animals at the time of LNCaP tumour challenge, HE3235 delayed the onset of the tumours and reduced tumour volumes in a dose-dependent manner, with a 100% inhibition of LNCaP tumours in the highest dose group. When treatment was applied to mice with existing tumours, a reduction in tumour volume was observed by the end of the first week of treatment, and tumour growth continued to be depressed throughout the course of the study. In addition, two out of nine animals treated with HE3235, in this study, had no detectable tumour volume by the end of the observation period. These results show that HE3235 had a cytoreductive effect on tumour volume.
Although the precise mechanism of action of HE3235 still remains to be elucidated, based on the in vitro and in vivo results presented here it would appear that HE3235 is not solely blocking the AED-stimulation of LNCaP tumours, rather, this anti-neoplastic activity seems to be through the induction of apoptosis. The exact mechanism through which HE3235 is inducing apoptosis is unclear. The observation that HE3235 did not inhibit the proliferation of PC3 and DU145 cells would suggest that the presence of the AR is required for its activity.
It is well established that DHT can cause a G1 arrest, but not cell death of LNCaP cells at concentrations above 10−10 nM (Tsihlias et al, 2000). Based on the results of the ARE studies, an experiment was conducted to determine whether HE3235 had a similar cytostatic effect on LNCaP. We also sought to determine whether, unlike DHT, exposure to HE3235 caused apoptosis in LNCaP cells. The results of these experiments showed that HE3235 does induce a modest increase in apoptosis. This finding would be consistent with the reduction in tumour volumes seen in mice treated with HE325. These effects are reminiscent of other agents reported to block the proliferation of prostate cancer cells in vitro and in vivo, most notable the histone deactylase inhibitors (HDACi). Using the LNCaP tumour model, sodium butyrate caused G1 cell-cycle arrest, inhibited LNCaP cell growth and increased PSA gene expression (Kruh, 1982; Gleave et al, 1998). Similar findings with respect to inhibition of cell proliferation and PSA secretion have been reported for vitamin D3 (Esquenet et al, 1996), calcitriol (Bauer et al, 2003), activin A, (Fujii et al, 2004) and phenylacetate (Walls et al, 1996). Future work will be directed towards further understanding whether the molecular mechanism of action of HE3235 is associated with an HDACi activity.
For many decades, androgen suppression has been the central theme for treating recurrent prostate cancer, yet virtually no patients are cured by this therapy. Prostate cancer progresses from androgen-dependent (AD) tumours that respond favourably to androgen ablation, to castration-resistant metastatic tumours that are invariably fatal. Pharmacological methods of lowering androgen levels in patients include the use of hormones that decrease androgen production, agents that block androgen association with the androgen receptor, and inhibitors of enzymes involved in androgen synthesis. Nonetheless, androgen receptor signalling remains active in castration-resistant prostate cancer despite these attempts at suppression. There are currently very few treatment options for men with castration-resistant prostate cancer. Androgen deprivation therapy induces a remission in 80–90% of men with advanced prostate cancer, and can increase median progression-free survival by 21 months (Hellerstedt and Pienta, 2002; Suzuki et al, 2007). This approach eventually fails to stop the disease progression because prostate tumour cells evolve to a castration-resistant state, in which tumours develop the ability to grow in castrate levels of T and DHT at least in part owing to the presence of AED. As described here, HE3235 represents a therapy that blocks the growth of AED-stimulated tumour cell growth, and thus, could be considered as a candidate for the treatment of castration-resistant prostate cancer.
|Bauer JA,Thompson TA,Church DR,Ariazi EA,Wilding G. Growth inhibition and differentiation in human prostate carcinoma cells induced by the vitamin D analog 1alpha, 24-dihydroxyvitamin D2Prostate 2003;55:159–167. [pmid: 12692781]|
|Cantor A. Extending SAS Survival Analysis Techniques for Medical Research 1997SAS Institute Inc.; :59–61.|
|Chang HC,Miyamoto H,Marwah P,Lardy H,Yeh S,Huang KE,Chang C. Suppression of Delta(5)-androstenediol-induced androgen receptor transactivation by selective steroids in human prostate cancer cellsProc Natl Acad Sci USA 1999;96:11173–11177.|
|Esquenet M,Swinnen JV,Heyns W,Verhoeven G. Control of LNCaP proliferation and differentiation: actions and interactions of androgens, 1alpha, 25-dihydroxycholecalciferol, all-trans retinoic acid, 9-cis retinoic acid, and phenylacetateProstate 1996;28:182–194. [pmid: 8628721]|
|Fujii Y,Kawakami S,Okada Y,Kageyama Y,Kihara K. Regulation of prostate-specific antigen by activin A in prostate cancer LNCaP cellsAm J Physiol Endocrinol Metab 2004;286:E927–E931. [pmid: 14761877]|
|Gleave ME,Sato N,Sadar M,Yago V,Bruchovsky N,Sullivan L. Butyrate analogue, isobutyramide, inhibits tumor growth and time to androgen-independent progression in the human prostate LNCaP tumor modelJ Cell Biochem 1998;69:271–281. [pmid: 9581866]|
|He D,Falany CN. Inhibition of SULT2B1b expression alters effects of 3beta-hydroxysteroids on cell proliferation and steroid hormone receptor expression in human LNCaP prostate cancer cellsProstate 2007;67:1318–1329. [pmid: 17626250]|
|Hellerstedt BA,Pienta KJ. The current state of hormonal therapy for prostate cancerCA Cancer J Clin 2002;52:154–179. [pmid: 12018929]|
|Kruh J. Effects of sodium butyrate, a new pharmacological agent, on cells in cultureMol Cell Biochem 1982;42:65–82. [pmid: 6174854]|
|Maggiolini M,Recchia AG,Carpino A,Vivacqua A,Fasanella G,Rago V,Pezzi V,Briand PA,Picard D,Ando S. Oestrogen receptor beta is required for androgen-stimulated proliferation of LNCaP prostate cancer cellsJ Mol Endocrinol 2004;32:777–791. [pmid: 15171712]|
|Miyamoto H,Yeh S,Lardy H,Messing E,Chang C. Delta5-androstenediol is a natural hormone with androgenic activity in human prostate cancer cellsProc Natl Acad Sci USA 1998;95:11083–11088.|
|Mizokami A,Koh E,Fujita H,Maeda Y,Egawa M,Koshida K,Honma S,Keller ET,Namiki M. The adrenal androgen androstenediol is present in prostate cancer tissue after androgen deprivation therapy and activates mutated androgen receptorCancer Res 2004;64:765–771. [pmid: 14744796]|
|Scher HI,Buchanan G,Gerald W,Butler LM,Tilley WD. Targeting the androgen receptor: improving outcomes for castration-resistant prostate cancerEndocr Relat Cancer 2004;11:459–476. [pmid: 15369448]|
|StatXactStatistical software for exact nonparametric inference, User Manual (version 7) 2004Cytel Software Corporation: Cambridge, MA372p|
|Suzuki K,Nishiyama T,Hara N,Yamana K,Takahashi K,Labrie F. Importance of the intracrine metabolism of adrenal androgens in androgen-dependent prostate cancerProstate Cancer Prostatic Dis 2007;10 3:301–306. [pmid: 17387321]|
|Taplin ME,Bubley GJ,Shuster TD,Frantz ME,Spooner AE,Ogata GK,Keer HN,Balk SP. Mutation of the androgen-receptor gene in metastatic androgen-independent prostate cancerN Engl J Med 1995;332:1393–1398. [pmid: 7723794]|
|Tsihlias J,Zhang W,Bhattacharya N,Flanagan M,Klotz L,Slingerland J. Involvement of p27Kip1 in G1 arrest by high dose 5 alpha-dihydrotestosterone in LNCaP human prostate cancer cellsOncogene 2000;19:670–679. [pmid: 10698512]|
|Walls R,Thibault A,Liu L,Wood C,Kozlowski JM,Figg WD,Sampson ML,Elin RJ,Samid D. The differentiating agent phenylacetate increases prostate-specific antigen production by prostate cancer cellsProstate 1996;29:177–182. [pmid: 8827086]|
|Westfall PH. Multiple comparisons and multiple tests 1999SAS Institute Inc.: Cary, NC, USA; :29–30.|
Keywords: prostate cancer, LNcaP, apoptosis.
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