Document Detail


Inhibition of anandamide hydrolysis by the enantiomers of ibuprofen, ketorolac, and flurbiprofen.
MedLine Citation:
PMID:  9989926     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The endogenous cannabimimetic anandamide is hydrolyzed by a fatty acid amide hydrolase to yield arachidonic acid and ethanolamine. In the present study, the regional distribution of the activity and its sensitivity to inhibition by the enantiomers of ibuprofen, ketorolac, and flurbiprofen has been investigated. The rate of [3H]anandamide hydrolysis was found in both 7-week-old and 90-week-old rats to be in the order hippocampus > cerebral cortex > cerebellum > striatum approximately midbrain, with higher rates of hydrolysis for the 7-week-old rats than for the 90-week-old rats. In whole brain (minus cerebellum), the R(-)-enantiomer of ibuprofen was a mixed-type inhibitor of anandamide hydrolysis and was approximately 2-3 times more potent than the S(+)-enantiomer, IC50 values of 230 and 750 microM, respectively, being found. A similar pattern of inhibition of anandamide hydrolysis was seen when intact C6 rat glioma cells were used. Ketorolac inhibited rat brain anandamide hydrolysis, with IC50 values of 50, 440, and 80 microM being found for the R-, S-, and R,S-forms, respectively. The IC50 value for R-flurbiprofen (60 microM) was similar to the IC50 value for the S-enantiomer (50 microM). These data demonstrate that there is no dramatic enantiomeric selectivity of NSAID compounds as inhibitors of fatty acid amide hydrolase enzyme(s) responsible for the hydrolysis of anandamide. The enantiomers of flurbiprofen and R-ketorolac are the most potent NSAID inhibitors of fatty acid amide hydrolase yet reported.
Authors:
C J Fowler; U Janson; R M Johnson; G Wahlström; A Stenström; K Norström; G Tiger
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  362     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-03-11     Completed Date:  1999-03-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  191-6     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Academic Press.
Affiliation:
Department of Pharmacology, Umeå University, Sweden. cf@pharm.umu.se
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MeSH Terms
Descriptor/Qualifier:
Aging
Animals
Arachidonic Acids / metabolism*
Brain / drug effects*,  metabolism,  pathology
Cell Extracts
Cell Survival / drug effects
Dose-Response Relationship, Drug
Flurbiprofen / analogs & derivatives,  pharmacology*
Glioma
Hydrolysis / drug effects
Ibuprofen / analogs & derivatives,  pharmacology*
Inhibitory Concentration 50
Ketorolac
Kinetics
Polyunsaturated Alkamides
Rats
Rats, Sprague-Dawley
Stereoisomerism
Time Factors
Tolmetin / analogs & derivatives*,  pharmacology
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Arachidonic Acids; 0/Cell Extracts; 0/Polyunsaturated Alkamides; 15687-27-1/Ibuprofen; 26171-23-3/Tolmetin; 5104-49-4/Flurbiprofen; 66635-83-4/Ketorolac; 94421-68-8/anandamide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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