| Inhibition of amyloid-beta-induced neurotoxicity and apoptosis by moderate ethanol preconditioning. | |
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MedLine Citation:
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PMID: 15486488 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Consumers of moderate amounts of ethanol have a lower risk of Alzheimer's dementia than do abstainers. In Alzheimer's disease the brain contains many extracellular plaques composed of amyloid-beta (Abeta), a neurotoxic protein linked to pathogenesis of the disease. Here we report that moderate ethanol preconditioning (20-30 mM for 6 days) of organotypic hippocampal-entorhinal slice cultures prevents Abeta-induced neurotoxicity and apoptosis as measured by media lactate dehydrogenase levels and staining with propidium iodide and Hoechst 33342. With Abeta, as with our previous studies of the neurotoxic HIV-1 protein gp120, moderate ethanol preconditioning may interfere with various glial-mediated neurotoxic responses in the slices to Abeta. In addition, we found that moderate ethanol preconditioning causes an almost 3-fold increase in brain levels of heat shock protein 70 (hsp70), a protective molecular chaperone. Our results suggest possible molecular mechanisms underlying the protective effect of moderate drinking against Alzheimer's dementia. |
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Authors:
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Abdelhak Belmadani; Sripathirathan Kumar; Matthew Schipma; Michael A Collins; Edward J Neafsey |
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Publication Detail:
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Type: Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Neuroreport Volume: 15 ISSN: 0959-4965 ISO Abbreviation: Neuroreport Publication Date: 2004 Sep |
Date Detail:
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Created Date: 2004-10-15 Completed Date: 2005-01-13 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9100935 Medline TA: Neuroreport Country: England |
Other Details:
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Languages: eng Pagination: 2093-6 Citation Subset: IM |
Affiliation:
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Division of Biochemistry, Department of Cell Biology, Neurobiology and Anatomy, Loyola University Medical Center, 2160 S. First Avenue, Maywood, IL 60153, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amyloid beta-Protein
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toxicity* Animals Animals, Newborn Apoptosis / drug effects* Benzimidazoles / diagnostic use Central Nervous System Depressants / pharmacology*, therapeutic use Dose-Response Relationship, Drug Drug Interactions Enzyme-Linked Immunosorbent Assay / methods Ethanol / pharmacology*, therapeutic use HSP70 Heat-Shock Proteins / metabolism Hippocampus / drug effects* Hydro-Lyases / metabolism Nerve Degeneration / etiology, prevention & control* Organ Culture Techniques Propidium / diagnostic use Rats Rats, Sprague-Dawley |
| Grant Support | |
ID/Acronym/Agency:
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AA11723/AA/NIAAA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amyloid beta-Protein; 0/Benzimidazoles; 0/Central Nervous System Depressants; 0/HSP70 Heat-Shock Proteins; 23491-52-3/HOE 33342; 36015-30-2/Propidium; 64-17-5/Ethanol; EC 4.2.1.-/Hydro-Lyases; EC 4.2.1.54/lactate dehydratase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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