| Inhibition and amplification of the radiation-induced degradation of DNA in Escherichia coli. | |
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MedLine Citation:
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PMID: 1090347 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The degradation of DNA in a repair-proficient strain (B/r) and in two repair-defective strains (Bs-1 and pol A) of Escherichia coli was studied in the presence and absence of three metabolic inhibitors after exposure of the cells to X-radiation. The radiation-induced degradation of DNA was dependent on energy production in freshly harvested log-phase cells and was closely related to the repair capacity of the cells. A different system, probably involving nonspecific deoxyribonucleases acting at radiation-induced strand breaks, appeared to be responsible for the degradative processes in stationary-phase cells or in log-phase cells which had been aged by standing in buffered saline for 2 days before irradiation. The three inhibitors tested (caffeine, rifampin, and carbonyl cyanide m-chlorophenyl hydrazone) were all found to inhibit partially the radiation-induced degradation of DNA in E. coli cells. Under other conditions, the same three compounds amplified the degradative process. The data suggested that the amplification was due to a selective inhibition of repair processes. The degradative processes which were stimulated by X-radiation of E. coli cells show many parallels to those which are evoked by phleomycin or colicine. |
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Authors:
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D K Myers |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Canadian journal of microbiology Volume: 21 ISSN: 0008-4166 ISO Abbreviation: Can. J. Microbiol. Publication Date: 1975 Jan |
Date Detail:
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Created Date: 1975-06-09 Completed Date: 1975-06-09 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 0372707 Medline TA: Can J Microbiol Country: CANADA |
Other Details:
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Languages: eng Pagination: 27-33 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Caffeine
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pharmacology* DNA Repair DNA, Bacterial / biosynthesis, metabolism*, radiation effects Dose-Response Relationship, Radiation Energy Metabolism Escherichia coli / growth & development, metabolism* Hydrazones Mutation Nitriles Phenylhydrazines / analogs & derivatives*, pharmacology Radiation Effects* Rifampin / pharmacology* Thymidine / metabolism Tritium |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/Hydrazones; 0/Nitriles; 0/Phenylhydrazines; 10028-17-8/Tritium; 13292-46-1/Rifampin; 50-89-5/Thymidine; 58-08-2/Caffeine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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