Document Detail


Inhibition of amino acid transport by ammonium ion in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  240806     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The rate of transport of L-amino acids by Saccharomyces cerevisiae epsilon 1278b increased with time in response to nitrogen starvation. This increase could be prevented by the addition of ammonium sulfate or cycloheximide. A slow time-dependent loss of transport activity was observed when ammonium sulfate (or ammonium sulfate plus cycloheximide) was added to cells after 3 h of nitrogen starvation. This loss of activity was not observed in the presence of cycloheximide alone. In a mutant yeast strain which lacks the nicotinamide adenine dinucleotide phosphate-dependent (anabolic) glutamate dehydrogenase, no significant decrease in amino acid transport was observed when ammonium sulfate was added to nitrogen-starved cells. A double mutant, which lacks the nicotinamide adenine dinucleotide phosphate-dependent enzyme and in addition has a depressed level of the nicotinamide adenine dinucleotide-dependent (catabolic) glutamate dehydrogenase, shows the same sensitivity to ammonium ion as the wild-type strain. These data suggest that the inhibition of amino acid transport by ammonium ion results from the uptake of this metabolite into the cell and its subsequent incorporation into the alpha-amino groups of glutamate and other amino acids.
Authors:
R J Roon; F Larimore; J S Levy
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  124     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1975 Oct 
Date Detail:
Created Date:  1975-12-29     Completed Date:  1975-12-29     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  325-31     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism*
Ammonium Sulfate / pharmacology*
Biological Transport, Active / drug effects
Cycloheximide / pharmacology
Depression, Chemical
Glutamate Dehydrogenase / biosynthesis
Mutation
NADP / metabolism
Nitrogen / metabolism
Saccharomyces cerevisiae / enzymology,  metabolism*
Stereoisomerism
Chemical
Reg. No./Substance:
0/Amino Acids; 53-59-8/NADP; 66-81-9/Cycloheximide; 7727-37-9/Nitrogen; 7783-20-2/Ammonium Sulfate; EC 1.4.1.2/Glutamate Dehydrogenase
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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