Document Detail

Inhibition of alkaline phosphatase activity by okadaic acid, a protein phosphatase inhibitor.
MedLine Citation:
PMID:  14505819     Owner:  NLM     Status:  MEDLINE    
This paper reports on a potential physiological target of okadaic acid (OA), the toxin metabolite responsible for shellfish poisoning and, consequently, human intoxication. OA is a potent promoter of tumor activity, most likely by inhibiting protein phosphatase 1 and 2A (Adv. Cancer. Res. 61 (1993) 143). However, all of its cellular targets have not yet been characterized. The interaction of OA with alkaline phosphatase (ALP) has been investigated in view of its protein phosphatase inhibition activity. Kinetic analysis of ALP from Escherichia coli, human placental and calf intestinal ALP has shown that OA acts as a non-competitive inhibitor of ALP. The bacterial enzyme displays a higher affinity for OA (K(i) 360 nM) than the eukaryotic proteins (human placental ALP, K(i) 2.05 microM; calf intestinal ALP, K(i) 3.15 microM). The inhibition by OA suggests a putative role of ALP in the phosphorylation status, through regulation of the phosphorylation/dephosphorylation equilibrium of proteins with phosphoseryl or phosphothreonyl residues.
V Mestrović; M Pavela-Vrancic
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimie     Volume:  85     ISSN:  0300-9084     ISO Abbreviation:  Biochimie     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-09-24     Completed Date:  2004-06-01     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  1264604     Medline TA:  Biochimie     Country:  France    
Other Details:
Languages:  eng     Pagination:  647-50     Citation Subset:  IM    
Department of Chemistry, Faculty of Natural Sciences, Mathematics and Education, University of Split, N. Tesle 12, 21000, Split, Croatia.
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MeSH Terms
Alkaline Phosphatase / metabolism*
Enzyme Inhibitors / pharmacology*
Escherichia coli / enzymology
Okadaic Acid / pharmacology*
Phosphoprotein Phosphatases / antagonists & inhibitors
Protein Phosphatase 1
Reg. No./Substance:
0/Enzyme Inhibitors; 78111-17-8/Okadaic Acid; EC Phosphatase; EC Phosphatases; EC Phosphatase 1

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