Document Detail


Inhibition of alkaline phosphatase activity by okadaic acid, a protein phosphatase inhibitor.
MedLine Citation:
PMID:  14505819     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This paper reports on a potential physiological target of okadaic acid (OA), the toxin metabolite responsible for shellfish poisoning and, consequently, human intoxication. OA is a potent promoter of tumor activity, most likely by inhibiting protein phosphatase 1 and 2A (Adv. Cancer. Res. 61 (1993) 143). However, all of its cellular targets have not yet been characterized. The interaction of OA with alkaline phosphatase (ALP) has been investigated in view of its protein phosphatase inhibition activity. Kinetic analysis of ALP from Escherichia coli, human placental and calf intestinal ALP has shown that OA acts as a non-competitive inhibitor of ALP. The bacterial enzyme displays a higher affinity for OA (K(i) 360 nM) than the eukaryotic proteins (human placental ALP, K(i) 2.05 microM; calf intestinal ALP, K(i) 3.15 microM). The inhibition by OA suggests a putative role of ALP in the phosphorylation status, through regulation of the phosphorylation/dephosphorylation equilibrium of proteins with phosphoseryl or phosphothreonyl residues.
Authors:
V Mestrović; M Pavela-Vrancic
Related Documents :
15280359 - Crystal structure and mutagenesis of a protein phosphatase-1:calcineurin hybrid elucida...
2965059 - Molecular cloning of cdna for human prostatic acid phosphatase.
1333409 - Phosphoprotein phosphatase 2a dephosphorylates eif-4e and does not alter binding to the...
20097759 - The neurosecretory vesicle protein phogrin functions as a phosphatidylinositol phosphat...
10585469 - Brain actin-associated protein phosphatase 1 holoenzymes containing spinophilin, neurab...
16696949 - Cloning and expression of maize-leaf pyruvate, pi dikinase regulatory protein gene.
10435589 - Titration of cellular export factors, but not heteromultimerization, is the molecular m...
20668909 - Spider silk proteins: recent advances in recombinant production, structure-function rel...
11315969 - Electron microscopic in situ hybridization study of simultaneous expression of tnp1 and...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimie     Volume:  85     ISSN:  0300-9084     ISO Abbreviation:  Biochimie     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-09-24     Completed Date:  2004-06-01     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  1264604     Medline TA:  Biochimie     Country:  France    
Other Details:
Languages:  eng     Pagination:  647-50     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Faculty of Natural Sciences, Mathematics and Education, University of Split, N. Tesle 12, 21000, Split, Croatia.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase / metabolism*
Animals
Enzyme Inhibitors / pharmacology*
Escherichia coli / enzymology
Humans
Kinetics
Okadaic Acid / pharmacology*
Phosphoprotein Phosphatases / antagonists & inhibitors
Protein Phosphatase 1
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 78111-17-8/Okadaic Acid; EC 3.1.3.1/Alkaline Phosphatase; EC 3.1.3.16/Phosphoprotein Phosphatases; EC 3.1.3.16/Protein Phosphatase 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Fine tuning the myosin motor: the role of the essential light chain in striated muscle myosin.
Next Document:  Plasmodium falciparum SERA protein peptide analogues having short helical regions induce protection ...